Human-mouse hybrids carrying fragments of single human chromosomes selected by tumor growth

D J Porteous, M M Wilkinson, J M Fletcher, V van Heyningen

Research output: Contribution to journalArticlepeer-review

Abstract / Description of output

Fusion of human EJ bladder carcinoma cells to mouse C127 cells, with direct selection for tumor growth, gave rise to hybrid cells in which the human chromosome complement had been reduced dramatically, while selectively retaining the activated HRAS1 at chromosome band 11p15. A single-component hybrid retaining only part of human chromosome 11 is described in detail. Our results suggest a novel and general approach for investigating the chromosomal basis of neoplastic change and for subchromosomal mapping of and enrichment cloning for the human genome.
Original languageEnglish
Pages (from-to)680-4
Number of pages5
JournalGenomics
Volume5
Issue number4
Publication statusPublished - 1989

Keywords / Materials (for Non-textual outputs)

  • Animals
  • Carcinoma
  • Cell Division
  • Cell Fusion
  • Chromosomes, Human, Pair 11
  • DNA, Neoplasm
  • Gene Expression
  • Genes, ras
  • Genetic Markers
  • Humans
  • Hybrid Cells
  • Mice
  • Tumor Cells, Cultured
  • Urinary Bladder Neoplasms

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