Human oestrogen receptors: differential expression of ER alpha and beta and the identification of ER beta variants

Graeme A Scobie, Sheila Macpherson, Michael R Millar, Nigel P Groome, Panagiota G Romana, Philippa T K Saunders

Research output: Contribution to journalArticlepeer-review

Abstract / Description of output

Two structurally related subtypes of oestrogen receptor (ER), known as alpha (ER alpha, NR3A1) and beta (ER beta, NR3A2) have been identified. ER beta mRNA and protein have been detected in a wide range of tissues including the vasculature, bone, and gonads in both males and females, as well as in cancers of the breast and prostate. In many tissues the pattern of expression of ER beta is distinct from that of ER alpha. A number of variant isoforms of the wild type beta receptor (ER beta 1), have been identified. In the human these include: (1). use of alternative start sites within the mRNA leading to translation of either a long (530 amino acids, hER beta 1L) or a truncated form (487aa hER beta 1s); (2). deletion of exons by alternative splicing; (3). formation of several isoforms (ER beta 2-beta 5) due to alternative splicing of exons encoding the carboxy terminus (F domain). We have raised monoclonal antibodies specific for hER beta1 as well as to three of the C terminal isoforms (beta2, beta 4 and beta 5). Using these antibodies we have found that ER beta 2, beta 4 and beta 5 proteins are expressed in nuclei of human tissues including the ovary, placenta, testis and vas deferens. In conclusion, in addition to the differential expression of full length ER alpha and ER beta a number of ER variant isoforms have been identified. The impact of the expression of these isoforms on cell responsiveness to oestrogens may add additional complexity to the ways in which oestrogenic ligands influence cell function.
Original languageEnglish
Pages (from-to)985-92
Number of pages8
JournalSteroids
Volume67
Issue number12
Publication statusPublished - 2002

Fingerprint

Dive into the research topics of 'Human oestrogen receptors: differential expression of ER alpha and beta and the identification of ER beta variants'. Together they form a unique fingerprint.

Cite this