Human papillomavirus mRNA and p16 detection as biomarkers for the improved diagnosis of cervical neoplasia

Kate Cuschieri, Nicolas Wentzensen

Research output: Contribution to journalArticlepeer-review

Abstract

UNLABELLED: Human papillomavirus (HPV) infection of the genital tract is very common and normally follows a benign clinical course; however, in an unfortunate minority of infected individuals, it can cause disease that sometimes leads to cancer. It is accepted that HPV DNA testing has a role in the management of cervical disease both in a prevaccination and postvaccination era; however, to improve the specificity of this approach, there is a requirement to develop and validate tools/assays that can identify women at risk for progressive disease. There is evidence to suggest that detection of viral gene expression both directly and indirectly may constitute a more specific approach for delineating clinically significant infection compared with HPV DNA-based assays. HPV oncogene expression and evidence of its deregulation can be monitored through direct detection of viral mRNA transcripts or through detection of the cellular protein p16. For both approaches, commercial assays have been introduced and numerous studies have been conducted. The present article describes the scientific theory underpinning these approaches, their amenability to routine-diagnostic specimens/settings, and the clinical data that has been garnered through their application thus far. Currently, there is promising data indicating that HPV mRNA and p16 might play an important role in future cervical cancer screening scenarios. Still, large randomized studies are necessary to confirm the preliminary data.

METHODS: PubMed and OVID were interrogated with search terms "HPV RNA;" "HPV mRNA;" "HPV transcript-detection, testing, and methods;" "p16" AND "cervical cancer;" "p16" AND "CIN;" "p16" AND "histology"; "p16" AND "cytology;" "p16;" and "screening."

Original languageEnglish
Pages (from-to)2536-45
Number of pages10
JournalCancer Epidemiology, Biomarkers and Prevention
Volume17
Issue number10
DOIs
Publication statusPublished - Oct 2008

Keywords

  • Female
  • Humans
  • Mass Screening
  • Neoplasm Proteins
  • Papillomaviridae
  • Papillomavirus Infections
  • Precancerous Conditions
  • RNA, Messenger
  • Tumor Markers, Biological
  • Uterine Cervical Neoplasms

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