Abstract / Description of output
The pygmy hippo is an Endangered mammal endemic to West Africa, of which only 2,000-3,000 are left in the wild. Until now genetic resources to conduct monitoring of wild populations and to facilitate captive breeding have been lacking. In this study we used restriction-site associated DNA sequencing of five pygmy hippo samples to generate 1,619 high confidence candidate single nucleotide polymorphisms (SNPs) suitable for population genetic analysis. A subset of 10 of SNPs generated were validated via resequencing with 100 % success rate and through the use of KASPar DNA probes (Kbiosciences) with 90 % success rate. To facilitate future research we present the list of 1,619 SNPs ranked according to mean genotype confidence and mean coverage.
Original language | English |
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Pages (from-to) | 535-538 |
Number of pages | 4 |
Journal | Conservation Genetics Resources |
Volume | 6 |
Issue number | 3 |
DOIs | |
Publication status | Published - 1 Jan 2014 |
Keywords / Materials (for Non-textual outputs)
- Conservation genetics
- Pygmy hippopotamus
- RAD sequencing
- Sequence data
- SNP discovery