Hybridization of electrodeposited magnetic multilayer micropillars

Justin J. Palfreyman, Frieda van Belle, Wen-Siang Lew, Thanos Mitrelias, J. A. C. Bland, Maria Lopalco, Mark Bradley

Research output: Contribution to journalArticlepeer-review

Abstract

A novel magnetic encoding technique for performing high-throughput biological assays using molecular probes such as antibodies and DNA oligonucleotides is presented. Electrodeposited Ni/Au multilayer pillar structures with diameters from 5-20 mu m and thicknesses from 1-10 mu m are presented as "magnetic barcodes," where the number of unique codes possible increases exponentially with a linear increase in pillar size. We have successfully functionalized these pillars using a HS-C-10-NHS self-assembled monolayer (SAM) grown on the An cap layer and attachment of an NH2 modified primer oligo sequence and have shown hybridization and denaturation to a fluorescent template. In addition we have developed a novel release layer methodology that allows a large library of probes to be inkjet printed on a single 5 mm(2) chip prior to release.

Original languageEnglish
Pages (from-to)2439-2441
Number of pages3
JournalIEEE Transactions on Magnetics
Volume43
Issue number6
DOIs
Publication statusPublished - Jun 2007

Keywords

  • DNA
  • electrodeposition
  • fluorescence
  • hybridization
  • magnetic barcodes
  • microarrays
  • microfluidics
  • MICROARRAY TECHNOLOGY

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