Projects per year
Abstract
Limiting dysfunctional neutrophilic inflammation whilst preserving effective immunity requires a better understanding of the processes that dictate neutrophil function in the tissues. Quantitative mass-spectrometry identified how inflammatory murine neutrophils regulated expression of cell surface receptors, signal transduction networks and metabolic machinery to shape neutrophil phenotypes in response to hypoxia. Through the tracing of labelled amino acids into metabolic enzymes, pro-inflammatory mediators and granule proteins we demonstrated that ongoing protein synthesis shapes the neutrophil proteome. To maintain energy supplies in the tissues, neutrophils consumed extracellular proteins to fuel central carbon metabolism. The physiological stresses of hypoxia and hypoglycaemia, characteristic of inflamed tissues, promoted this extra-cellular protein scavenging with activation of the lysosomal compartment further driving exploitation of the protein rich inflammatory milieu. This study provides a comprehensive map of neutrophil proteomes, analysis of which has led to the identification of active catabolic and anabolic pathways which enable neutrophils to sustain synthetic and effector functions in the tissues.
Original language | English |
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Journal | Journal of Clinical Investigation |
Early online date | 6 Apr 2021 |
DOIs | |
Publication status | E-pub ahead of print - 6 Apr 2021 |
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Dive into the research topics of 'Hypoxia drives murine neutrophil protein scavenging to maintain central carbon metabolism'. Together they form a unique fingerprint.Projects
- 2 Finished
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Defining the interplay between hypoxia and metabolic adaptations of the innate immune response.
Walmsley, S. (Principal Investigator)
1/01/18 → 31/12/22
Project: Research
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Transfer from Sheffield: Regulation or neutrophilic inflammation by the HIF/PHD pathway
Walmsley, S. (Principal Investigator)
1/09/14 → 31/12/17
Project: Research
Equipment
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Institute for Genetics and Cancer Mass Spectrometry Facility
Von Kriegsheim, A. (Manager)
Deanery of Molecular, Genetic and Population Health SciencesFacility/equipment: Facility