Identification and characterization of a homozygous deletion found in ovarian ascites by representational difference analysis

J E Watson, H Gabra, K J Taylor, G J Rabiasz, H Morrison, P Perry, J F Smyth, D J Porteous

Research output: Contribution to journalArticlepeer-review

Abstract / Description of output

We have performed representational difference analysis (RDA) on DNA from tumor cells and normal fibroblasts isolated from the ascites of a patient with ovarian cancer. Five of six products of the RDA were homozygously deleted from the tumor DNA. One of these products has been characterized and identifies a homozygous deletion of approximately 6.9 Mb at chromosome 9p21 in the original ovarian tumor material. This deletion encompasses CDKN2A (p16), CDKN2B (p15), and IFN-alpha. PCR analysis of other tumor cell lines using the novel STS based on the RDA product has shown it to lie between IFN-alpha and p16, and to identify the distal extent of a homozygous deletion in another ovarian cancer cell line. These data provide further evidence for a tumor suppressor locus distinct from, but mapping close to, p16 on 9p21. Cytogenetic analysis using comparative genomic hybridization (CGH) performed on the same primary tumor confirmed a loss of material from chromosome 9p. However, the CGH technique had neither the resolution nor the sensitivity to define a subregion of homozygous loss.
Original languageEnglish
Pages (from-to)226-33
Number of pages8
JournalGenome Research
Volume9
Issue number3
Publication statusPublished - 1999

Keywords / Materials (for Non-textual outputs)

  • Ascites
  • Cells, Cultured
  • Chromosome Mapping
  • Chromosomes, Human, Pair 9
  • DNA Mutational Analysis
  • DNA, Neoplasm
  • Female
  • Genes, Tumor Suppressor
  • Homozygote
  • Humans
  • Molecular Sequence Data
  • Nucleic Acid Hybridization
  • Ovarian Neoplasms
  • Sequence Deletion
  • Tumor Cells, Cultured

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