Identification and characterization of novel factors that act in the nonsense‐mediated mRNA decay pathway in nematodes, flies and mammals

Angela Casadio, Dasa Longman, Nele Hug, Laurent Delavaine, Raúl Vallejos Baier, Claudio R Alonso, Javier F Cáceres

Research output: Contribution to journalArticlepeer-review

Abstract

Nonsense‐mediated mRNA decay (NMD) is a surveillance mechanism that degrades mRNAs harboring premature termination codons (PTCs). We have conducted a genome‐wide RNAi screen in Caenorhabditis elegans that resulted in the identification of five novel NMD genes that are conserved throughout evolution. Two of their human homologs, GNL2 (ngp‐1) and SEC13 (npp‐20), are also required for NMD in human cells. We also show that the C. elegans gene noah‐2, which is present in Drosophila melanogaster but absent in humans, is an NMD factor in fruit flies. Altogether, these data identify novel NMD factors that are conserved throughout evolution, highlighting the complexity of the NMD pathway and suggesting that yet uncovered novel factors may act to regulate this process.

Original languageEnglish
Pages (from-to)71-78
JournalEMBO Reports
Volume16
Issue number1
Early online date1 Dec 2014
DOIs
Publication statusPublished - 1 Jan 2015

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