Identification and characterization of novel factors that act in the nonsense‐mediated mRNA decay pathway in nematodes, flies and mammals

Angela Casadio; Dasa Longman; Nele Hug; Laurent Delavaine; Raúl Vallejos Baier; Claudio R Alonso; Javier F Cáceres

doi:10.15252/embr.201439183

Identification and characterization of novel factors that act in the nonsense‐mediated mRNA decay pathway in nematodes, flies and mammals

Angela Casadio, Dasa Longman, Nele Hug, Laurent Delavaine, Raúl Vallejos Baier, Claudio R Alonso, Javier F Cáceres

Research output: Contribution to journal › Article › peer-review

Abstract / Description of output

Nonsense‐mediated mRNA decay (NMD) is a surveillance mechanism that degrades mRNAs harboring premature termination codons (PTCs). We have conducted a genome‐wide RNAi screen in Caenorhabditis elegans that resulted in the identification of five novel NMD genes that are conserved throughout evolution. Two of their human homologs, GNL2 (ngp‐1) and SEC13 (npp‐20), are also required for NMD in human cells. We also show that the C. elegans gene noah‐2, which is present in Drosophila melanogaster but absent in humans, is an NMD factor in fruit flies. Altogether, these data identify novel NMD factors that are conserved throughout evolution, highlighting the complexity of the NMD pathway and suggesting that yet uncovered novel factors may act to regulate this process.

Original language	English
Pages (from-to)	71-78
Journal	EMBO Reports
Volume	16
Issue number	1
Early online date	1 Dec 2014
DOIs	https://doi.org/10.15252/embr.201439183
Publication status	Published - 1 Jan 2015

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10.15252/embr.201439183Licence: Creative Commons: Attribution (CC-BY)

Identification and characterization of novel factors that act in the nonsense‐mediated mRNA decay pathway in nematodes, flies and mammals
This is an open access article under the terms of the Creative Commons Attribution 4.0 License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
Final published version, 963 KBLicence: Creative Commons: Attribution (CC-BY)

CONTROL OF TRANSLATION ELONGATION FACTO eEF1A ISOFORM SWITCHING
Abbott, C.
UK-based charities
1/06/11 → 31/07/14
Project: Research

Dasa Longman
- Deanery of Molecular, Genetic and Population Health Sciences - Senior Scientist
- MRC Human Genetics Unit
Person: Academic: Research Active , Academic: Research Active (Research Assistant)

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title = "Identification and characterization of novel factors that act in the nonsense‐mediated mRNA decay pathway in nematodes, flies and mammals",

abstract = "Nonsense‐mediated mRNA decay (NMD) is a surveillance mechanism that degrades mRNAs harboring premature termination codons (PTCs). We have conducted a genome‐wide RNAi screen in Caenorhabditis elegans that resulted in the identification of five novel NMD genes that are conserved throughout evolution. Two of their human homologs, GNL2 (ngp‐1) and SEC13 (npp‐20), are also required for NMD in human cells. We also show that the C. elegans gene noah‐2, which is present in Drosophila melanogaster but absent in humans, is an NMD factor in fruit flies. Altogether, these data identify novel NMD factors that are conserved throughout evolution, highlighting the complexity of the NMD pathway and suggesting that yet uncovered novel factors may act to regulate this process.",

author = "Angela Casadio and Dasa Longman and Nele Hug and Laurent Delavaine and {Vallejos Baier}, Ra{\'u}l and Alonso, {Claudio R} and C{\'a}ceres, {Javier F}",

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AU - Casadio, Angela

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AU - Hug, Nele

AU - Delavaine, Laurent

AU - Vallejos Baier, Raúl

AU - Alonso, Claudio R

AU - Cáceres, Javier F

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AB - Nonsense‐mediated mRNA decay (NMD) is a surveillance mechanism that degrades mRNAs harboring premature termination codons (PTCs). We have conducted a genome‐wide RNAi screen in Caenorhabditis elegans that resulted in the identification of five novel NMD genes that are conserved throughout evolution. Two of their human homologs, GNL2 (ngp‐1) and SEC13 (npp‐20), are also required for NMD in human cells. We also show that the C. elegans gene noah‐2, which is present in Drosophila melanogaster but absent in humans, is an NMD factor in fruit flies. Altogether, these data identify novel NMD factors that are conserved throughout evolution, highlighting the complexity of the NMD pathway and suggesting that yet uncovered novel factors may act to regulate this process.

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Identification and characterization of novel factors that act in the nonsense‐mediated mRNA decay pathway in nematodes, flies and mammals

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CONTROL OF TRANSLATION ELONGATION FACTO eEF1A ISOFORM SWITCHING

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Dasa Longman

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