Projects per year
Abstract / Description of output
Every organism has a different set of genes essential for its viability. This indicates that an organism can become tolerant to the loss of an essential gene under certain circumstances during evolution, via the manifestation of ‛masked' alternative mechanisms. In our quest to systematically uncover masked mechanisms in eukaryotic cells, we developed an extragenic suppressor screening method using haploid spores deleted of an essential gene in the fission yeast Schizosaccharomyces pombe. We screened for the ‛bypass' suppressors of lethality of 92 randomly selected genes that are essential for viability in standard laboratory culture conditions. Remarkably, extragenic mutations bypassed the essentiality of as many as 20 genes (22%), 15 of which have not been previously reported. Half of the bypass-suppressible genes were involved in mitochondria function; we also identified multiple genes regulating RNA processing. 18 suppressible genes were conserved in the budding yeast Saccharomyces cerevisiae, but 13 of them were non-essential in that species. These trends suggest that essentiality bypass is not a rare event and that each organism may be endowed with secondary or backup mechanisms that can substitute for primary mechanisms in various biological processes. Furthermore, the robustness of our simple spore-based methodology paves the way for genome-scale screening. Keywords: Schizosaccharomyces pombe, essential gene, extragenic suppressor screening.
Original language | English |
---|---|
Pages (from-to) | 113-119 |
Journal | Cell structure and function |
Volume | 44 |
Issue number | 2 |
Early online date | 31 Aug 2019 |
DOIs | |
Publication status | E-pub ahead of print - 31 Aug 2019 |
Keywords / Materials (for Non-textual outputs)
- Schizosaccharomyces pombe
- extragenic suppressor screening
- bypass of essentiality (BOE)
- cut7 (kinesin-5)
- hul5 (E3 ubiquitin ligase)
Fingerprint
Dive into the research topics of 'Identification of 15 new bypassable essential genes of fission yeast'. Together they form a unique fingerprint.Projects
- 2 Finished
-
Regulation of fission yeast cell polarity by stress-signalling pathways
1/09/18 → 31/08/23
Project: Research
-
Regulation of microtubule nucleation by Mto1/2 complex and the gamma-tubulin complex
11/04/12 → 10/04/18
Project: Research