Identification of a cDNA clone for mouse apoprotein A-1 (apo A-1) and its use in characterization of apo A-1 mRNA expression in liver and small intestine

J C Miller, R K Barth, P H Shaw, R W Elliott, N D Hastie

Research output: Contribution to journalArticlepeer-review

Abstract / Description of output

A cDNA clone for mouse apoprotein A-1 (apo A-1), the major apoprotein of plasma high density lipoproteins, has been identified. In addition to structural and physiological evidence, a genetic polymorphism for mouse plasma apo A-1 has been used to confirm that this DNA sequence corresponds to the apo A-1 gene. Use of this clone in molecular hybridization studies has shown that the concentration of apo A-1 mRNA is similar in liver and small intestine and is constant along the entire length of the small intestine. We provide evidence that the same apo A-1 gene is expressed in both liver and small intestine. Apo A-1 mRNA is also present in the stomach and esophagus at 10-15% the concentration found in small intestine but is undetectable in other tissues (such as large intestine, pancreas, heart, kidney, spleen, and brain). Finally, we show that there is a differential effect of a diet high in saturated fat and cholesterol on apo A-1 mRNA levels in liver and small intestine.

Original languageEnglish
Pages (from-to)1511-5
Number of pages5
JournalProceedings of the National Academy of Sciences (PNAS)
Volume80
Issue number6
Publication statusPublished - Mar 1983

Keywords / Materials (for Non-textual outputs)

  • Animals
  • Apolipoproteins
  • Apolipoproteins A
  • Cholesterol
  • Cloning, Molecular
  • DNA
  • Dietary Fats
  • Gene Expression Regulation
  • Intestine, Small
  • Liver
  • Mice
  • Polymorphism, Genetic
  • RNA, Messenger

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