Identification of a dominant negative functional domain on DAPK-1 that degrades DAPK-1 protein and stimulates TNFR-1-mediated apoptosis

Yao Lin, Craig Stevens, Ted Hupp

Research output: Contribution to journalArticlepeer-review

Abstract

DAPK-1 is a stress-activated tumor suppressor protein that plays a role in both proapoptotic or antiapoptotic signal transduction pathways. To define mechanisms of DAPK-1 protein regulation, we have determined that DAPK-1 protein has a long half-life, and therefore its activity is primarily regulated at the protein level. Changes in DAPK-1 protein levels occur by a cathepsin B-dependent pathway, prompting us to evaluate whether cathepsin B plays positive or negative role in DAPK-1 function. The transfection of p55-TNFR-1 induced complex formation between DAPK-1 and cathepsin B. Depletion of cathepsin B protein using small interfering RNA stimulated TNFR-1 dependent apoptosis. The minimal binding region on DAPK-1 for cathepsin B was mapped to amino acids 836-947. The transfection of the DAPK-1-(836-947) miniprotein acted in a dominant negative manner inducing endogenous DAPK-1 protein degradation in a TNFR-1-dependent manner. These data suggest that DAPK-1 forms a multiprotein survival complex with cathepsin B countering the rate of TNFR-1-dependent apoptosis and highlights the importance of developing DAPK-1 inhibitors as agents to sensitize cells to stress-induced apoptosis.
Original languageEnglish
Pages (from-to)16792-802
Number of pages11
JournalJournal of Biological Chemistry
Volume282
Issue number23
DOIs
Publication statusPublished - 8 Jun 2007

Keywords

  • Apoptosis
  • Apoptosis Regulatory Proteins
  • Base Sequence
  • Calcium-Calmodulin-Dependent Protein Kinases
  • Cell Line
  • DNA Primers
  • Death-Associated Protein Kinases
  • Fluorescent Antibody Technique
  • Humans
  • Hydrolysis
  • Immunoprecipitation
  • RNA, Small Interfering
  • Receptors, Tumor Necrosis Factor, Type I

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