Projects per year
Abstract
Nonsense-mediated decay (NMD) is a translation-dependent RNA quality control mechanism that occurs in the cytoplasm. However, it is unknown how NMD regulates the stability of RNAs translated at the endoplasmic reticulum (ER). Here, we identify a localized NMD pathway dedicated to ER-translated mRNAs. We previously identified NBAS, a component of the Syntaxin 18 complex involved in Golgi-to-ER trafficking, as a novel NMD factor. Furthermore, we show that NBAS fulfills an independent function in NMD. This ER–NMD pathway requires the interaction of NBAS with the core NMD factor UPF1, which is partially localized at the ER in the proximity of the translocon. NBAS and UPF1 coregulate the stability of ER-associated transcripts, in particular those associated with the cellular stress response. We propose a model where NBAS recruits UPF1 to the membrane of the ER and activates an ER-dedicated NMD pathway, thus providing an ER-protective function by ensuring quality control of ER-translated mRNAs.
Original language | English |
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Pages (from-to) | 1075-1088 |
Journal | Genes & Development |
Volume | 34 |
Issue number | 15-16 |
Early online date | 2 Jul 2020 |
DOIs | |
Publication status | E-pub ahead of print - 2 Jul 2020 |
Keywords / Materials (for Non-textual outputs)
- nonsense-mediated decay (NMD)
- RNA quality control
- UPF1
- NBAS
- ER stress
- UPR
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Dive into the research topics of 'Identification of a localized Nonsense-Mediated Decay pathway at the Endoplasmic Reticulum'. Together they form a unique fingerprint.Projects
- 2 Finished
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Post-transcriptional regulation of Gene Expression
Caceres, J. (Principal Investigator)
1/04/18 → 31/03/23
Project: Research
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Mutagenesis and its Biological Impact
Taylor, M. (Principal Investigator)
1/04/18 → 31/03/23
Project: Research
Equipment
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Advanced Imaging Resource
Wheeler, A. (Manager), Murphy, L. (Other), Lee, M. (Other), Caldwell, H. (Other), Pearson, M. (Other) & Iremonger, J. (Other)
Deanery of Molecular, Genetic and Population Health SciencesFacility/equipment: Facility