Abstract
Expression of an esterase gene from Streptomyces scabies is regulated by zinc in both Streptomyces scabies and Streptomyces lividans. A specific protein-binding site was identified on an esterase promoter fragment by using an S-30 extract from S. scabies. The location of the protein-binding site was determined by gel shift assays of promoter deletion fragments and by DNase I footprinting analysis. The protein-binding site maps from nucleotides -59 to -81 relative to the start of transcription. An esterase gene construct cloned and expressed in S. lividans was used to assess the importance of the protein-binding site. Deletion of the 23-bp protein-binding site resulted in a 10-fold decrease in esterase production when cells were grown in zinc-inducing conditions. The protein-binding site may represent a region involved in positive regulation of the S. scabies esterase gene.
Original language | English |
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Pages (from-to) | 4287-93 |
Number of pages | 7 |
Journal | Journal of Bacteriology |
Volume | 174 |
Issue number | 13 |
Publication status | Published - Jul 1992 |
Keywords
- Amino Acid Sequence
- Base Sequence
- Binding Sites
- Chromosome Deletion
- Cloning, Molecular
- DNA, Bacterial
- Deoxyribonuclease I
- Esterases
- Gene Expression Regulation, Bacterial
- Gene Expression Regulation, Enzymologic
- Genes, Bacterial
- Kinetics
- Molecular Sequence Data
- Mutagenesis, Site-Directed
- Oligodeoxyribonucleotides
- Protein Binding
- Restriction Mapping
- Streptomyces
- Transcription, Genetic
- Transformation, Bacterial