TY - JOUR
T1 - Identification of Altered Evoked and Non-Evoked Responses in a Heterologous Mouse Model of Endometriosis-Associated Pain
AU - Tejada, Miguel A.
AU - Santos-llamas, Ana I.
AU - Escriva, Lesley
AU - Tarin, Juan J.
AU - Cano, Antonio
AU - Fernández-ramírez, Maria J.
AU - Nunez-badinez, Paulina
AU - De Leo, Bianca
AU - Saunders, Philippa T. K.
AU - Vidal, Victor
AU - Barthas, Florent
AU - Vincent, Katy
AU - Sweeney, Patrick J.
AU - Sillito, Rowland R.
AU - Armstrong, James Douglas
AU - Nagel, Jens
AU - Gomez, Raúl
N1 - Funding Information:
Funding: This project has received funding from the Innovative Medicines Initiative 2 Joint Undertaking under grant agreement no. (777500). This Joint Undertaking receives support from the European Union’s Horizon 2020 research and innovation program and EFPIA. Further information is found under www.imi-paincare.eu (accessed on 16 February 2022) and www.imi.europa.eu (accessed on 16 February 2022). Additional funding was supported by the Spanish Ministry of Economy and Competitiveness through the Miguel Servet Program (CPII18/00037 (2018)) cofounded by FEDER (European Regional Development Fund) awarded to Raul Gómez as well as by Carlos III Institute of Health grants (PI17/02329 (2017) and PI20/01835 (2020)) awarded to Raul Gómez and predoctoral PFIS FI18/00322 (2018) awarded to Ana Santos.
Publisher Copyright:
© 2022 by the authors. Licensee MDPI, Basel, Switzerland.
PY - 2022/2/21
Y1 - 2022/2/21
N2 - The aim of this study was to develop and refine a heterologous mouse model of endometriosis-associated pain in which non-evoked responses, more relevant to the patient experience, were evaluated. Immunodeficient female mice (N = 24) were each implanted with four endometriotic human lesions (N = 12) or control tissue fat (N = 12) on the abdominal wall using tissue glue. Evoked pain responses were measured biweekly using von Frey filaments. Non-evoked responses were recorded weekly for 8 weeks using a home cage analysis (HCA). Endpoints were distance traveled, social proximity, time spent in the center vs. outer areas of the cage, drinking, and climbing. Significant differences between groups for von Frey response, climbing, and drinking were detected on days 14, 21, and 35 post implanting surgery, respectively, and sustained for the duration of the experiment. In conclusion, a heterologous mouse model of endometriosis-associated evoked a non-evoked pain was developed to improve the relevance of preclinical models to patient experience as a platform for drug testing.
AB - The aim of this study was to develop and refine a heterologous mouse model of endometriosis-associated pain in which non-evoked responses, more relevant to the patient experience, were evaluated. Immunodeficient female mice (N = 24) were each implanted with four endometriotic human lesions (N = 12) or control tissue fat (N = 12) on the abdominal wall using tissue glue. Evoked pain responses were measured biweekly using von Frey filaments. Non-evoked responses were recorded weekly for 8 weeks using a home cage analysis (HCA). Endpoints were distance traveled, social proximity, time spent in the center vs. outer areas of the cage, drinking, and climbing. Significant differences between groups for von Frey response, climbing, and drinking were detected on days 14, 21, and 35 post implanting surgery, respectively, and sustained for the duration of the experiment. In conclusion, a heterologous mouse model of endometriosis-associated evoked a non-evoked pain was developed to improve the relevance of preclinical models to patient experience as a platform for drug testing.
U2 - 10.3390/biomedicines10020501
DO - 10.3390/biomedicines10020501
M3 - Article
SN - 2227-9059
VL - 10
SP - 501
JO - Biomedicines
JF - Biomedicines
IS - 2
ER -