Identification of an antigen specific to Trypanosoma congolense by using monoclonal antibodies

N M Parish, Ivan Morrison, T. W. Pearson

Research output: Contribution to journalArticlepeer-review

Abstract

Using indirect immunofluorescence assays on acetone-fixed smears of a series of different parasites, we have shown that two monoclonal antibodies bind specifically to Trypanosoma congolense organisms. The antibodies bind to both bloodstream trypomastigotes and procyclic culture forms of the parasite and are thus not stage specific. Immunoprecipitation and immunoblot analysis showed that both monoclonal reagents bound a protein of approximately 31,000 m.w. This antigen appeared to be located on the plasma membrane of T. congolense, but the epitope was not exposed on the surface of living bloodstream or procyclic organisms. The antigen was detectable on acetone-fixed organisms or in trypanosome lysates in enzyme-linked immunosorbent assays and may therefore by useful as a species-specific marker in field assays for epidemiologic and clinical investigations.
Original languageEnglish
Pages (from-to)593-7
Number of pages5
JournalJournal of Immunology
Volume134
Issue number1
Publication statusPublished - Jan 1985

Keywords

  • Animals
  • Antibodies, Monoclonal
  • Antigen-Antibody Complex
  • Antigens/analysis
  • Enzyme-Linked Immunosorbent Assay
  • Female
  • Fluorescent Antibody Technique
  • Mice
  • Mice, Inbred BALB C
  • Species Specificity
  • Trypanosoma/immunology
  • Trypanosoma congolense/immunology
  • Trypanosomiasis, African/immunology

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