Identification of bacteriophage-encoded anti-sRNAs in pathogenic escherichia coli

Jai J Tree; Sander Granneman; Sean P McAteer; David Tollervey; David L Gally

doi:10.1016/j.molcel.2014.05.006

Identification of bacteriophage-encoded anti-sRNAs in pathogenic escherichia coli

Jai J Tree, Sander Granneman, Sean P McAteer, David Tollervey^*, David L Gally

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

Abstract

In bacteria, Hfq is a core RNA chaperone that catalyzes the interaction of mRNAs with regulatory small RNAs (sRNAs). To determine in vivo RNA sequence requirements for Hfq interactions, and to study riboregulation in a bacterial pathogen, Hfq was UV crosslinked to RNAs in enterohemorrhagic Escherichia coli (EHEC). Hfq bound repeated trinucleotide motifs of A-R-N (A-A/G-any nucleotide) often associated with the Shine-Dalgarno translation initiation sequence in mRNAs. These motifs overlapped or were adjacent to the mRNA sequences bound by sRNAs. In consequence, sRNA-mRNA duplex formation will displace Hfq, promoting recycling. Fifty-five sRNAs were identified within bacteriophage-derived regions of the EHEC genome, including some of the most abundant Hfq-interacting sRNAs. One of these (AgvB) antagonized the function of the core genome regulatory sRNA, GcvB, by mimicking its mRNA substrate sequence. This bacteriophage-encoded "anti-sRNA" provided EHEC with a growth advantage specifically in bovine rectal mucus recovered from its primary colonization site in cattle.

Original language	English
Pages (from-to)	199-213
Number of pages	15
Journal	Molecular Cell
Volume	55
Issue number	2
Early online date	5 Jun 2014
DOIs	https://doi.org/10.1016/j.molcel.2014.05.006
Publication status	Published - 17 Jul 2014

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10.1016/j.molcel.2014.05.006Licence: Creative Commons: Attribution (CC-BY)

Identification of Bacteriophage-Encoded Anti-sRNAs in Pathogenic Escherichia coliFinal published version, 5.78 MBLicence: Creative Commons: Attribution (CC-BY)

Innate immunity and endemic diseases in livestock species
Collie, D. (Principal Investigator), Beard, P. (Co-investigator), Bishop, S. (Co-investigator), Bronsvoort, M. (Co-investigator), Burt, D. (Co-investigator), Fitzgerald, R. (Co-investigator), Freeman, T. (Co-investigator), Gally, D. (Co-investigator), Gill, A. (Co-investigator), Glass, E. (Co-investigator), Hocking, P. (Co-investigator), Hope, J. (Co-investigator), Hume, D. (Co-investigator), Kaiser, P. (Co-investigator), Mabbott, N. (Co-investigator), McLachlan, G. (Co-investigator), Morrison, L. (Co-investigator), Stevens, J. (Co-investigator), Stevens, M. (Co-investigator) & Watson, M. (Co-investigator)
BBSRC
1/04/12 → 31/03/17
Project: Research

David Gally
- dgallyed.acuk
- Royal (Dick) School of Veterinary Studies - Personal Chair of Microbial Genetics
Person: Academic: Research Active

Cite this

@article{effe75e4e4ff41438c5791bb1961144b,

title = "Identification of bacteriophage-encoded anti-sRNAs in pathogenic escherichia coli",

abstract = "In bacteria, Hfq is a core RNA chaperone that catalyzes the interaction of mRNAs with regulatory small RNAs (sRNAs). To determine in vivo RNA sequence requirements for Hfq interactions, and to study riboregulation in a bacterial pathogen, Hfq was UV crosslinked to RNAs in enterohemorrhagic Escherichia coli (EHEC). Hfq bound repeated trinucleotide motifs of A-R-N (A-A/G-any nucleotide) often associated with the Shine-Dalgarno translation initiation sequence in mRNAs. These motifs overlapped or were adjacent to the mRNA sequences bound by sRNAs. In consequence, sRNA-mRNA duplex formation will displace Hfq, promoting recycling. Fifty-five sRNAs were identified within bacteriophage-derived regions of the EHEC genome, including some of the most abundant Hfq-interacting sRNAs. One of these (AgvB) antagonized the function of the core genome regulatory sRNA, GcvB, by mimicking its mRNA substrate sequence. This bacteriophage-encoded {"}anti-sRNA{"} provided EHEC with a growth advantage specifically in bovine rectal mucus recovered from its primary colonization site in cattle.",

author = "Tree, {Jai J} and Sander Granneman and McAteer, {Sean P} and David Tollervey and Gally, {David L}",

note = "Research at the Roslin Institute is supported by BBSRC Institute grant funding",

year = "2014",

month = jul,

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doi = "10.1016/j.molcel.2014.05.006",

language = "English",

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pages = "199--213",

journal = "Molecular Cell",

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T1 - Identification of bacteriophage-encoded anti-sRNAs in pathogenic escherichia coli

AU - Tree, Jai J

AU - Granneman, Sander

AU - McAteer, Sean P

AU - Tollervey, David

AU - Gally, David L

N1 - Research at the Roslin Institute is supported by BBSRC Institute grant funding

PY - 2014/7/17

Y1 - 2014/7/17

N2 - In bacteria, Hfq is a core RNA chaperone that catalyzes the interaction of mRNAs with regulatory small RNAs (sRNAs). To determine in vivo RNA sequence requirements for Hfq interactions, and to study riboregulation in a bacterial pathogen, Hfq was UV crosslinked to RNAs in enterohemorrhagic Escherichia coli (EHEC). Hfq bound repeated trinucleotide motifs of A-R-N (A-A/G-any nucleotide) often associated with the Shine-Dalgarno translation initiation sequence in mRNAs. These motifs overlapped or were adjacent to the mRNA sequences bound by sRNAs. In consequence, sRNA-mRNA duplex formation will displace Hfq, promoting recycling. Fifty-five sRNAs were identified within bacteriophage-derived regions of the EHEC genome, including some of the most abundant Hfq-interacting sRNAs. One of these (AgvB) antagonized the function of the core genome regulatory sRNA, GcvB, by mimicking its mRNA substrate sequence. This bacteriophage-encoded "anti-sRNA" provided EHEC with a growth advantage specifically in bovine rectal mucus recovered from its primary colonization site in cattle.

AB - In bacteria, Hfq is a core RNA chaperone that catalyzes the interaction of mRNAs with regulatory small RNAs (sRNAs). To determine in vivo RNA sequence requirements for Hfq interactions, and to study riboregulation in a bacterial pathogen, Hfq was UV crosslinked to RNAs in enterohemorrhagic Escherichia coli (EHEC). Hfq bound repeated trinucleotide motifs of A-R-N (A-A/G-any nucleotide) often associated with the Shine-Dalgarno translation initiation sequence in mRNAs. These motifs overlapped or were adjacent to the mRNA sequences bound by sRNAs. In consequence, sRNA-mRNA duplex formation will displace Hfq, promoting recycling. Fifty-five sRNAs were identified within bacteriophage-derived regions of the EHEC genome, including some of the most abundant Hfq-interacting sRNAs. One of these (AgvB) antagonized the function of the core genome regulatory sRNA, GcvB, by mimicking its mRNA substrate sequence. This bacteriophage-encoded "anti-sRNA" provided EHEC with a growth advantage specifically in bovine rectal mucus recovered from its primary colonization site in cattle.

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DO - 10.1016/j.molcel.2014.05.006

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SN - 1097-2765

VL - 55

SP - 199

EP - 213

JO - Molecular Cell

JF - Molecular Cell

IS - 2

ER -

Identification of bacteriophage-encoded anti-sRNAs in pathogenic escherichia coli

Abstract

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Innate immunity and endemic diseases in livestock species

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David Gally

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