Identification of cattle infected with bovine virus diarrhoea virus using a monoclonal antibody capture ELISA

A Fenton, P F Nettleton, Gary Entrican, J A Herring, C Malloy, A. Greig, Christopher Low

Research output: Contribution to journalArticlepeer-review

Abstract

A monoclonal antibody capture enzyme linked immunosorbent assay (ELISA) has been developed to detect pestivirus-specific antigen in the leucocytes of cattle infected with bovine virus diarrhoea virus (BVDV). A blind trial was conducted to compare the specificity of the ELISA with conventional tissue culture virus isolation on 215 blood samples submitted for BVDV diagnosis from cattle throughout Scotland. One hundred and sixty seven samples were negative by both ELISA and virus isolation and 47 samples were positive by both tests. One blood was negative by ELISA and positive by virus isolation.
Original languageEnglish
Pages (from-to)169-74
Number of pages6
JournalArchives of virology. Supplementum
Volume3
DOIs
Publication statusPublished - 1991

Keywords

  • animals
  • Antibodies, Monoclonal
  • Antigens, Viral
  • Bovine Virus Diarrhea-Mucosal Disease
  • Cattle
  • Diarrhea Viruses, Bovine Viral
  • Enzyme-Linked Immunosorbent Assay

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