Identification of disease markers by differential display: prion disease

Michael Clinton, Gino Miele, Sunil Nandi, Derek McBride

Research output: Contribution to journalArticlepeer-review

Abstract

In order to identify molecular markers of prion disease in peripheral tissues, we used the differential display reverse-transcriptase polymerase chain reaction (DDRT-PCR) procedure to compare gene expression in spleens of infected and uninfected mice. In this study, we identified a novel erythroid-specific gene that was differentially expressed as a result of prion infection. We were able to demonstrate that a decrease in the expression levels of this transcript in hematopoietic tissues was a common feature of prion diseases. Our findings suggest a previously unknown role for the blood erythroid lineage in the development of prion diseases and should provide a new focus for research into diagnostic and therapeutic strategies.
Original languageEnglish
Pages (from-to)157-78
Number of pages22
JournalMethods in Molecular Biology
Volume317
Publication statusPublished - 2006

Keywords

  • Animals
  • Biological Markers
  • Blotting, Northern
  • Blotting, Southern
  • Cattle
  • Cloning, Molecular
  • DNA
  • DNA, Complementary
  • Electrophoresis, Agar Gel
  • Encephalopathy, Bovine Spongiform
  • Erythroid Cells
  • Female
  • Gene Expression Profiling
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Nucleic Acid Hybridization
  • Polymerase Chain Reaction
  • Polymorphism, Single-Stranded Conformational
  • Prion Diseases
  • RNA
  • RNA, Messenger
  • Reverse Transcriptase Polymerase Chain Reaction
  • Spleen
  • Tissue Distribution

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