Identification of IL-10 competent B cells in swine.

Jemma V Milburn, Anna Hoog, Sonia Villanueva-Hernández, Kerstin H Mair, Wilhelm Gerner

Research output: Contribution to journalArticlepeer-review

Abstract / Description of output

Progress in the phenotypic characterisation of porcine B cells is ongoing, with recent advances in the identification of B1 cell subsets and plasma cells. However, regulatory B cells, commonly identified by interleukin (IL)-10 production, have not been studied in pigs so far. Here we investigate IL-10 expression in B cell subsets in response to CpG-oligodeoxynucleotides, phorbol 12-myristate 13-acetate and ionomycin stimulation in vitro. Our results reflect similar findings in human and mice. We identify a small subset of IL-10 competent B cells, present within both porcine B1 and B2 cell subsets across blood, spleen, mediastinal lymph nodes and lung tissue, with varied differentiation statuses. The capacity for IL-10 production coincided with CD95 expression, suggesting an activated phenotype of IL-10 competent B cells. These findings support the emerging paradigm that B cell IL-10 production is a function of various B cell subsets influenced by activation history and microenvironmental factors.

Original languageUndefined/Unknown
Pages (from-to)1-12
JournalDevelopmental and Comparative Immunology
Early online date28 Jun 2022
Publication statusPublished - Oct 2022

Keywords / Materials (for Non-textual outputs)

  • Breg
  • B10 cell
  • IL-10
  • Pig

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