Identification of immuno-reactive capsid proteins of malignant catarrhal fever viruses

Kathryn Bartley, David Deane, Ann Percival, Inga R. Dry, Dawn M. Grant, Neil F. Inglis, Kevin Mclean, Erin D T Manson, Lisa H J Imrie, David M. Haig, Felix Lankester, George C. Russell*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract / Description of output

Malignant catarrhal fever (MCF) is a fatal disease of cattle and other ungulates caused by certain gamma-herpesviruses including alcelaphine herpesvirus-1 (AlHV-1) and ovine herpesvirus-2 (OvHV-2). An attenuated virus vaccine based on AlHV-1 has been shown to induce virus-neutralising antibodies in plasma and nasal secretions of protected cattle but the targets of virus-specific antibodies are unknown. Proteomic analysis and western blotting of virus extracts allowed the identification of eight candidate AlHV-1 virion antigens. Recombinant expression of selected candidates and their OvHV-2 orthologues confirmed that two polypeptides, the products of the ORF17.5 and ORF65 genes, were antigens recognised by antibodies from natural MCF cases or from AlHV-1 vaccinated cattle. These proteins have potential as diagnostic and/or vaccine antigens.

Original languageEnglish
Pages (from-to)17-26
JournalVeterinary Microbiology
Volume173
Issue number1-2
Early online date18 Jul 2014
DOIs
Publication statusPublished - 2014

Keywords / Materials (for Non-textual outputs)

  • Antibodies
  • Antigen identification
  • Inflammatory disease
  • Malignant catarrhal fever
  • Proteomics
  • Recombinant expression

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