Identification of immuno-reactive capsid proteins of malignant catarrhal fever viruses

Kathryn Bartley; David Deane; Ann Percival; Inga R. Dry; Dawn M. Grant; Neil F. Inglis; Kevin Mclean; Erin D T Manson; Lisa H J Imrie; David M. Haig; Felix Lankester; George C. Russell

doi:10.1016/j.vetmic.2014.07.004

Identification of immuno-reactive capsid proteins of malignant catarrhal fever viruses

Kathryn Bartley, David Deane, Ann Percival, Inga R. Dry, Dawn M. Grant, Neil F. Inglis, Kevin Mclean, Erin D T Manson, Lisa H J Imrie, David M. Haig, Felix Lankester, George C. Russell^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

Abstract

Malignant catarrhal fever (MCF) is a fatal disease of cattle and other ungulates caused by certain gamma-herpesviruses including alcelaphine herpesvirus-1 (AlHV-1) and ovine herpesvirus-2 (OvHV-2). An attenuated virus vaccine based on AlHV-1 has been shown to induce virus-neutralising antibodies in plasma and nasal secretions of protected cattle but the targets of virus-specific antibodies are unknown. Proteomic analysis and western blotting of virus extracts allowed the identification of eight candidate AlHV-1 virion antigens. Recombinant expression of selected candidates and their OvHV-2 orthologues confirmed that two polypeptides, the products of the ORF17.5 and ORF65 genes, were antigens recognised by antibodies from natural MCF cases or from AlHV-1 vaccinated cattle. These proteins have potential as diagnostic and/or vaccine antigens.

Original language	English
Pages (from-to)	17-26
Journal	Veterinary Microbiology
Volume	173
Issue number	1-2
Early online date	18 Jul 2014
DOIs	https://doi.org/10.1016/j.vetmic.2014.07.004
Publication status	Published - 2014

Keywords

Antibodies
Antigen identification
Inflammatory disease
Malignant catarrhal fever
Proteomics
Recombinant expression

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10.1016/j.vetmic.2014.07.004

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@article{45779a81e91d45fc88455ac70793f26e,

title = "Identification of immuno-reactive capsid proteins of malignant catarrhal fever viruses",

abstract = "Malignant catarrhal fever (MCF) is a fatal disease of cattle and other ungulates caused by certain gamma-herpesviruses including alcelaphine herpesvirus-1 (AlHV-1) and ovine herpesvirus-2 (OvHV-2). An attenuated virus vaccine based on AlHV-1 has been shown to induce virus-neutralising antibodies in plasma and nasal secretions of protected cattle but the targets of virus-specific antibodies are unknown. Proteomic analysis and western blotting of virus extracts allowed the identification of eight candidate AlHV-1 virion antigens. Recombinant expression of selected candidates and their OvHV-2 orthologues confirmed that two polypeptides, the products of the ORF17.5 and ORF65 genes, were antigens recognised by antibodies from natural MCF cases or from AlHV-1 vaccinated cattle. These proteins have potential as diagnostic and/or vaccine antigens.",

keywords = "Antibodies, Antigen identification, Inflammatory disease, Malignant catarrhal fever, Proteomics, Recombinant expression",

author = "Kathryn Bartley and David Deane and Ann Percival and Dry, {Inga R.} and Grant, {Dawn M.} and Inglis, {Neil F.} and Kevin Mclean and Manson, {Erin D T} and Imrie, {Lisa H J} and Haig, {David M.} and Felix Lankester and Russell, {George C.}",

year = "2014",

doi = "10.1016/j.vetmic.2014.07.004",

language = "English",

volume = "173",

pages = "17--26",

journal = "Veterinary Microbiology",

issn = "0378-1135",

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AU - Bartley, Kathryn

AU - Deane, David

AU - Percival, Ann

AU - Dry, Inga R.

AU - Grant, Dawn M.

AU - Inglis, Neil F.

AU - Mclean, Kevin

AU - Manson, Erin D T

AU - Imrie, Lisa H J

AU - Haig, David M.

AU - Lankester, Felix

AU - Russell, George C.

PY - 2014

Y1 - 2014

N2 - Malignant catarrhal fever (MCF) is a fatal disease of cattle and other ungulates caused by certain gamma-herpesviruses including alcelaphine herpesvirus-1 (AlHV-1) and ovine herpesvirus-2 (OvHV-2). An attenuated virus vaccine based on AlHV-1 has been shown to induce virus-neutralising antibodies in plasma and nasal secretions of protected cattle but the targets of virus-specific antibodies are unknown. Proteomic analysis and western blotting of virus extracts allowed the identification of eight candidate AlHV-1 virion antigens. Recombinant expression of selected candidates and their OvHV-2 orthologues confirmed that two polypeptides, the products of the ORF17.5 and ORF65 genes, were antigens recognised by antibodies from natural MCF cases or from AlHV-1 vaccinated cattle. These proteins have potential as diagnostic and/or vaccine antigens.

AB - Malignant catarrhal fever (MCF) is a fatal disease of cattle and other ungulates caused by certain gamma-herpesviruses including alcelaphine herpesvirus-1 (AlHV-1) and ovine herpesvirus-2 (OvHV-2). An attenuated virus vaccine based on AlHV-1 has been shown to induce virus-neutralising antibodies in plasma and nasal secretions of protected cattle but the targets of virus-specific antibodies are unknown. Proteomic analysis and western blotting of virus extracts allowed the identification of eight candidate AlHV-1 virion antigens. Recombinant expression of selected candidates and their OvHV-2 orthologues confirmed that two polypeptides, the products of the ORF17.5 and ORF65 genes, were antigens recognised by antibodies from natural MCF cases or from AlHV-1 vaccinated cattle. These proteins have potential as diagnostic and/or vaccine antigens.

KW - Antibodies

KW - Antigen identification

KW - Inflammatory disease

KW - Malignant catarrhal fever

KW - Proteomics

KW - Recombinant expression

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DO - 10.1016/j.vetmic.2014.07.004

M3 - Article

VL - 173

SP - 17

EP - 26

JO - Veterinary Microbiology

JF - Veterinary Microbiology

SN - 0378-1135

IS - 1-2

ER -

Identification of immuno-reactive capsid proteins of malignant catarrhal fever viruses

Abstract

Keywords

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