TY - UNPB
T1 - Identification of MAZ as a novel transcription factor regulating erythropoiesis
AU - Deen, Darya
AU - Butter , Falk
AU - Holland, Michelle L.
AU - Samara, Vasiliki
AU - Sloane-Stanley, Jacqueline A.
AU - Ayyub, Helena
AU - Mann, Matthias
AU - Garrick, David
AU - Vernimmen, Douglas
PY - 2020/5/10
Y1 - 2020/5/10
N2 - Erythropoiesis requires a combination of ubiquitous and tissue-specific transcription factors. Here, through DNA affinity purification followed by mass spectrometry, we have identified the widely expressed protein MAZ (Myc-associated zinc finger) as a transcription factor that binds to the promoter of the erythroid-specific human α-globin gene. Genome-wide mapping in primary human erythroid cells revealed that MAZ also occupies active promoters as well as GATA1-bound enhancer elements of key erythroid genes. Consistent with an important role during erythropoiesis, knockdown of MAZ in primary human erythroid cells impairs erythroid differentiation, and genetic variants in the MAZ locus are associated with clinically important human erythroid traits. Taken together, these findings reveal the Zinc-finger transcription factor MAZ to be a previously unrecognised regulator of the erythroid differentiation program.
AB - Erythropoiesis requires a combination of ubiquitous and tissue-specific transcription factors. Here, through DNA affinity purification followed by mass spectrometry, we have identified the widely expressed protein MAZ (Myc-associated zinc finger) as a transcription factor that binds to the promoter of the erythroid-specific human α-globin gene. Genome-wide mapping in primary human erythroid cells revealed that MAZ also occupies active promoters as well as GATA1-bound enhancer elements of key erythroid genes. Consistent with an important role during erythropoiesis, knockdown of MAZ in primary human erythroid cells impairs erythroid differentiation, and genetic variants in the MAZ locus are associated with clinically important human erythroid traits. Taken together, these findings reveal the Zinc-finger transcription factor MAZ to be a previously unrecognised regulator of the erythroid differentiation program.
UR - https://www.biorxiv.org/content/10.1101/2020.05.10.087254v1
U2 - 10.1101/2020.05.10.087254
DO - 10.1101/2020.05.10.087254
M3 - Working paper
BT - Identification of MAZ as a novel transcription factor regulating erythropoiesis
ER -