Identification of negative and positive regulatory elements in the human renin gene

D W Burt, N Nakamura, P Kelley, V J Dzau

Research output: Contribution to journalArticlepeer-review

Abstract

Renin gene expression is tissue-specific and under complex hormonal control. To investigate which DNA elements are involved in the control of human renin gene expression, we performed transient DNA transfer experiments with renin-chloramphenicol acetyltransferase fusions. We have mapped a complex arrangement of positive and negative control sequences in the 5' flanking region of the human renin gene. One positive control element is active in either orientation and defines a renin gene enhancer. The negative element is also active in either orientation and defines a renin gene silencer. Mapping in the same region as the silencer is a cAMP-responsive element, a sequence conserved in mouse, rat, and human renin genes.
Original languageEnglish
Pages (from-to)7357-62
Number of pages6
JournalJournal of Biological Chemistry
Volume264
Issue number13
Publication statusPublished - 1989

Keywords

  • Animals
  • Base Sequence
  • Chloramphenicol O-Acetyltransferase/genetics
  • Choriocarcinoma
  • Cyclic AMP/pharmacology
  • Enhancer Elements, Genetic
  • Gene Expression Regulation/drug effects
  • Humans
  • Mice
  • Molecular Sequence Data
  • Promoter Regions, Genetic
  • Rats
  • Regulatory Sequences, Nucleic Acid
  • Renin/genetics
  • Sequence Homology, Nucleic Acid
  • Transcription, Genetic
  • Tumor Cells, Cultured

Fingerprint

Dive into the research topics of 'Identification of negative and positive regulatory elements in the human renin gene'. Together they form a unique fingerprint.

Cite this