Identification of noncoding transcripts from within CENP-A chromatin at fission yeast centromeres

E.S. Choi, A.G. Castillo, R.C. Allshire, A. Strålfors, M. Durand-Dubief, K. Ekwall

Research output: Contribution to journalArticlepeer-review

Abstract

The histone H3 variant CENP-A is the most favored candidate for an epigenetic mark that specifies the centromere. In fission yeast, adjacent heterochromatin can direct CENP-A chromatin establishment, but the underlying features governing where CENP-A chromatin assembles are unknown. We show that, in addition to centromeric regions, a low level of CENP-A associates with gene promoters where histone H3 is depleted by the activity of the Hrp1 chromatinremodeling factor. Moreover, we demonstrate that noncoding RNAs are transcribed by RNA polymerase II (RNAPII) from CENP-A chromatin at centromeres. These analyses reveal a similarity between centromeres and a subset of RNAPII genes and suggest a role for remodeling at RNAPII promoters within centromeres that influences the replacement of histone H3 with CENP-A.
Original languageEnglish
Pages (from-to)23600-23607
Number of pages8
JournalJournal of Biological Chemistry
Volume286
Issue number26
DOIs
Publication statusPublished - 1 Jul 2011

Keywords

  • Centromeres
  • Chromatin Remodeling
  • Nucleosome
  • Transcription
  • Yeast
  • CENP-A
  • Noncoding Transcripts

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