Identification of stage-specific changes in protein secretion by isolated seminiferous tubules from rats following exposure to short-term local testicular heating

TT McLaren, P M Foster, Richard M Sharpe

Research output: Contribution to journalArticlepeer-review

Abstract / Description of output

The objective of this study was to identify the early (after 4 and 24 h) effects of short-term local testicular heating (43 degrees C for 30 min) on the secretion of proteins by seminiferous tubules isolated from adult rats at stages II-V, VI-VIII or IX-XII of the spermatogenic cycle, and cultured in vitro for 24 h with [35S]methionine. Incorporation of [35S]methionine into secreted and intracellular proteins was assessed and the pattern of protein secretion was evaluated using two-dimensional SDS-PAGE. Seminiferous tubules isolated from control rats exhibited the characteristic, androgen-dependent increase in protein secretion at stages VI-VIII. At 4 h after exposure to local testicular heating, seminiferous tubules at these stages showed a significant increase (P < 0.001) in the overall incorporation of [35S]methionine into secreted proteins, whereas seminiferous tubules at stages II-V and IX-XII showed no significant change. In marked contrast, seminiferous tubules isolated from rats 24 h after local testicular heating showed a significant decrease in the incorporation of [35S]methionine into secreted proteins at stages VI-VIII (P < 0.001) and to a lesser extent at IX-XII (P < 0.05), whereas seminiferous tubules at stages II-V showed no change in incorporation. Prior treatment to maintain normal intratesticular concentrations of testosterone in heat-exposed rats failed to prevent these changes. Similar results were obtained when incorporation of [35S]methionine into intracellular proteins was evaluated 4 and 24 h after exposure to local testicular heating.(ABSTRACT TRUNCATED AT 250 WORDS)
Original languageEnglish
Pages (from-to)293-300
Number of pages8
JournalJournal of reproduction and fertility
Volume102
Issue number2
DOIs
Publication statusPublished - Nov 1994

Keywords / Materials (for Non-textual outputs)

  • Animals
  • Culture Techniques
  • Electrophoresis, Gel, Two-Dimensional
  • Hot Temperature/adverse effects
  • Male
  • Methionine
  • PROTEINS
  • RATS
  • Rats, Wistar
  • Seminiferous tubules
  • SPERMATOGENESIS
  • Time factors

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