An improved protocol has been developed for physical enrichment of cDNA sequences by hybridization to genomic DNA. When applied to microdissection recombinants derived from a translocation breakpoint region associated with inherited mental illness, a single cycle of the procedure permitted enriched cDNAs to be visualized directly by agarose gel electrophoresis. Hybridization screening of a library of clones derived from the enriched cDNAs, employing the genomic resource as a probe, led to the identification of six novel gene fragments. This general approach to the isolation of regionally encoded genes could be applied to any subchromosomal interval as a first step towards global transcription map construction.
|Number of pages||6|
|Publication status||Published - 1995|
- Animals Base Sequence Blotting, Southern Brain/*metabolism Callithrix Cats Cattle *Chromosomes, Human, Pair 1 Cricetinae DNA, Complementary/genetics Dogs Electrophoresis, Agar Gel Gene Library Humans Mice Molecular Sequence Data *Nucleic Acid Hybridization Polymerase Chain Reaction Psychotic Disorders/*genetics Rabbits Rats Sheep Species Specificity Swine *Translocation, Genetic