Identifying genes within microdissected genomic DNA: isolation of brain expressed genes from a translocation region associated with inherited mental illness

A. J. Brookes, E. M. Slorach, Kathryn Evans, M. L. Thomson, C. M. Gosden, W. J. Muir, D. J. Porteous

Research output: Contribution to journalArticlepeer-review

Abstract

An improved protocol has been developed for physical enrichment of cDNA sequences by hybridization to genomic DNA. When applied to microdissection recombinants derived from a translocation breakpoint region associated with inherited mental illness, a single cycle of the procedure permitted enriched cDNAs to be visualized directly by agarose gel electrophoresis. Hybridization screening of a library of clones derived from the enriched cDNAs, employing the genomic resource as a probe, led to the identification of six novel gene fragments. This general approach to the isolation of regionally encoded genes could be applied to any subchromosomal interval as a first step towards global transcription map construction.
Original languageEnglish
Pages (from-to)257-262
Number of pages6
JournalMammalian Genome
Volume6
Issue number4
Publication statusPublished - 1995

Keywords

  • Animals Base Sequence Blotting, Southern Brain/*metabolism Callithrix Cats Cattle *Chromosomes, Human, Pair 1 Cricetinae DNA, Complementary/genetics Dogs Electrophoresis, Agar Gel Gene Library Humans Mice Molecular Sequence Data *Nucleic Acid Hybridization Polymerase Chain Reaction Psychotic Disorders/*genetics Rabbits Rats Sheep Species Specificity Swine *Translocation, Genetic

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