Imaging Conditioned Fear Circuitry Using Awake Rodent fMRI

Nichola M Brydges, Heather C Whalley, Maurits A Jansen, Gavin D Merrifield, Emma R Wood, Stephen M Lawrie, Sara-Madge Wynne, Mark Day, Susan Fleetwood-Walker, Douglas Steele, Ian Marshall, Jeremy Hall, Megan C Holmes

Research output: Contribution to journalArticlepeer-review

Abstract / Description of output

Functional magnetic resonance imaging (fMRI) is a powerful method for exploring emotional and cognitive brain responses in humans. However rodent fMRI has not previously been applied to the analysis of learned behaviour in awake animals, limiting its use as a translational tool. Here we have developed a novel paradigm for studying brain activation in awake rats responding to conditioned stimuli using fMRI. Using this method we show activation of the amygdala and related fear circuitry in response to a fear-conditioned stimulus and demonstrate that the magnitude of fear circuitry activation is increased following early life stress, a rodent model of affective disorders. This technique provides a new translatable method for testing environmental, genetic and pharmacological manipulations on emotional and cognitive processes in awake rodent models.
Original languageEnglish
Article numbere54197
Number of pages7
JournalPLoS ONE
Issue number1
Publication statusPublished - Jan 2013


Dive into the research topics of 'Imaging Conditioned Fear Circuitry Using Awake Rodent fMRI'. Together they form a unique fingerprint.

Cite this