Abstract / Description of output

Extrachromosomal DNA (ecDNA) are circular regions of DNA that are found in many cancers.
They are an important means of oncogene amplification, and correlate with treatment resistance
and poor prognosis. Consequently, there is great interest in exploring and targeting ecDNA
vulnerabilities as potential new therapeutic targets for cancer treatment. However, the
biological significance of ecDNA and their associated regulatory control remains unclear.
Light microscopy has been a central tool in the identification and characterisation of ecDNA.
In this review we describe the different cellular models available to study ecDNA, and the
imaging tools used to characterise ecDNA and their regulation. The insights gained from
quantitative imaging are discussed in comparison with genome sequencing and computational
approaches. We suggest that there is a crucial need for ongoing innovation using imaging if we
are to achieve a full understanding of the dynamic regulation and organisation of ecDNA and
their role in tumourigenesis.
Original languageEnglish
JournalHistochemistry and cell biology
Publication statusPublished - 16 Apr 2024


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