Imaging extrachromosomal DNA (ecDNA) in cancer

Karin Purshouse; Steven M Pollard; Wendy A Bickmore

Imaging extrachromosomal DNA (ecDNA) in cancer

Karin Purshouse, Steven M Pollard, Wendy A Bickmore^*

^*Corresponding author for this work

Research output: Contribution to journal › Review article › peer-review

Abstract

Extrachromosomal DNA (ecDNA) are circular regions of DNA that are found in many cancers.
They are an important means of oncogene amplification, and correlate with treatment resistance
and poor prognosis. Consequently, there is great interest in exploring and targeting ecDNA
vulnerabilities as potential new therapeutic targets for cancer treatment. However, the
biological significance of ecDNA and their associated regulatory control remains unclear.
Light microscopy has been a central tool in the identification and characterisation of ecDNA.
In this review we describe the different cellular models available to study ecDNA, and the
imaging tools used to characterise ecDNA and their regulation. The insights gained from
quantitative imaging are discussed in comparison with genome sequencing and computational
approaches. We suggest that there is a crucial need for ongoing innovation using imaging if we
are to achieve a full understanding of the dynamic regulation and organisation of ecDNA and
their role in tumourigenesis.

Original language	English
Journal	Histochemistry and cell biology
Publication status	Published - 16 Apr 2024

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Imaging Extrachromosomal DNA_rev_track_changesAccepted author manuscript, 2.16 MBLicence: Creative Commons: Attribution (CC-BY)
s00418-024-02280-2Final published version, 0.98 MBLicence: Creative Commons: Attribution (CC-BY)

1 Finished
1 Active

Enhancer function and dysfunction: molecular mechanisms, genome context, and disease
Bickmore, W. (Principal Investigator)
1/04/23 → 31/03/28
Project: Research
MC_UU_00007/2 The role of spatial nuclear organisation in genome function
Bickmore, W. (Principal Investigator)
1/04/18 → 1/04/23
Project: Research

Cite this

@article{7bfbd033877a43be9b05a2ecd92a9884,

title = "Imaging extrachromosomal DNA (ecDNA) in cancer",

abstract = "Extrachromosomal DNA (ecDNA) are circular regions of DNA that are found in many cancers. They are an important means of oncogene amplification, and correlate with treatment resistance and poor prognosis. Consequently, there is great interest in exploring and targeting ecDNA vulnerabilities as potential new therapeutic targets for cancer treatment. However, the biological significance of ecDNA and their associated regulatory control remains unclear. Light microscopy has been a central tool in the identification and characterisation of ecDNA. In this review we describe the different cellular models available to study ecDNA, and the imaging tools used to characterise ecDNA and their regulation. The insights gained from quantitative imaging are discussed in comparison with genome sequencing and computational approaches. We suggest that there is a crucial need for ongoing innovation using imaging if we are to achieve a full understanding of the dynamic regulation and organisation of ecDNA and their role in tumourigenesis.",

author = "Karin Purshouse and Pollard, {Steven M} and Bickmore, {Wendy A}",

year = "2024",

month = apr,

day = "16",

language = "English",

journal = "Histochemistry and cell biology",

issn = "0948-6143",

publisher = "Springer",

}

TY - JOUR

T1 - Imaging extrachromosomal DNA (ecDNA) in cancer

AU - Purshouse, Karin

AU - Pollard, Steven M

AU - Bickmore, Wendy A

PY - 2024/4/16

Y1 - 2024/4/16

N2 - Extrachromosomal DNA (ecDNA) are circular regions of DNA that are found in many cancers. They are an important means of oncogene amplification, and correlate with treatment resistance and poor prognosis. Consequently, there is great interest in exploring and targeting ecDNA vulnerabilities as potential new therapeutic targets for cancer treatment. However, the biological significance of ecDNA and their associated regulatory control remains unclear. Light microscopy has been a central tool in the identification and characterisation of ecDNA. In this review we describe the different cellular models available to study ecDNA, and the imaging tools used to characterise ecDNA and their regulation. The insights gained from quantitative imaging are discussed in comparison with genome sequencing and computational approaches. We suggest that there is a crucial need for ongoing innovation using imaging if we are to achieve a full understanding of the dynamic regulation and organisation of ecDNA and their role in tumourigenesis.

AB - Extrachromosomal DNA (ecDNA) are circular regions of DNA that are found in many cancers. They are an important means of oncogene amplification, and correlate with treatment resistance and poor prognosis. Consequently, there is great interest in exploring and targeting ecDNA vulnerabilities as potential new therapeutic targets for cancer treatment. However, the biological significance of ecDNA and their associated regulatory control remains unclear. Light microscopy has been a central tool in the identification and characterisation of ecDNA. In this review we describe the different cellular models available to study ecDNA, and the imaging tools used to characterise ecDNA and their regulation. The insights gained from quantitative imaging are discussed in comparison with genome sequencing and computational approaches. We suggest that there is a crucial need for ongoing innovation using imaging if we are to achieve a full understanding of the dynamic regulation and organisation of ecDNA and their role in tumourigenesis.

M3 - Review article

SN - 0948-6143

JO - Histochemistry and cell biology

JF - Histochemistry and cell biology

ER -

Imaging extrachromosomal DNA (ecDNA) in cancer

Abstract

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Projects

Enhancer function and dysfunction: molecular mechanisms, genome context, and disease

MC_UU_00007/2 The role of spatial nuclear organisation in genome function

Cite this