Imaging plasmodesmata

Karen Bell, Karl Oparka

Research output: Contribution to journalArticlepeer-review

Abstract

At only 50 nm in diameter, plasmodesmata (PD) are below the limit of resolution of conventional light microscopy. Consequently, much of our current interpretation of the substructure of PD is derived from transmission electron microscopy. However, PD can be imaged with alternative techniques, including field emission scanning electron microscopy and 'super-resolution' imaging approaches such as 3D-structured illumination microscopy. This review considers the methods currently available for studying PD and focuses on the boundary between light- and electron-based imaging approaches.
Original languageEnglish
Pages (from-to)9-25
Number of pages17
JournalProtoplasma
Volume248
Issue number1
Early online date12 Nov 2010
DOIs
Publication statusPublished - 31 Jan 2011

Keywords / Materials (for Non-textual outputs)

  • Plasmodesmata
  • Transmission electron microscopy
  • 3D-structured illumination microscopy

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