Abstract
At only 50 nm in diameter, plasmodesmata (PD) are below the limit of resolution of conventional light microscopy. Consequently, much of our current interpretation of the substructure of PD is derived from transmission electron microscopy. However, PD can be imaged with alternative techniques, including field emission scanning electron microscopy and 'super-resolution' imaging approaches such as 3D-structured illumination microscopy. This review considers the methods currently available for studying PD and focuses on the boundary between light- and electron-based imaging approaches.
Original language | English |
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Pages (from-to) | 9-25 |
Number of pages | 17 |
Journal | Protoplasma |
Volume | 248 |
Issue number | 1 |
Early online date | 12 Nov 2010 |
DOIs | |
Publication status | Published - 31 Jan 2011 |
Keywords / Materials (for Non-textual outputs)
- Plasmodesmata
- Transmission electron microscopy
- 3D-structured illumination microscopy