Imaging sporozoite cell traversal in the liver of mice

Joana Tavares, Pauline Formaglio, Alexander Medvinsky, Robert Ménard, Rogerio Amino

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Intravital fluorescence microscopy is an invaluable tool to study a dynamic phenomenon through its direct observation in living organisms. This technique can combine qualitative and quantitative analysis and has been capital to address long-standing questions about Plasmodium biology. Beyond a descriptive view of the parasite life cycle, the possibility to image infection in transgenic animals in which a specific cell type, molecule or process is labeled opens new possibilities to study host cell-parasite interactions in cellular and molecular details. An additional layer of refinement can be achieved with the use of fluorescent knockout mutants (parasite, mice, or both) to dissect the molecular basis of the process of interest. Here, we present a basic protocol for imaging the sporozoite behavior in the liver, emphasizing the detection of the sporozoite's ability to traverse host cells.

Original languageEnglish
Title of host publicationMalaria
Subtitle of host publicationMethods and Protocols
PublisherHUMANA PRESS INC
Pages401-410
Number of pages10
ISBN (Electronic)978-1-62703-026-7
ISBN (Print)9781627030250
DOIs
Publication statusPublished - 2013

Publication series

NameMethods in Molecular Biology
Volume923
ISSN (Print)1064-3745

Keywords

  • cell traversal
  • endothelial barrier
  • in vivo imaging
  • malaria
  • spinning-disk

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