Imidazolium-tagged glycan probes for non-covalent labeling of live cells

David Benito-alifonso; Shirley Tremell; Joanna C. Sadler; Monica Berry; M. Carmen Galan

doi:10.1039/C5CC10040B

Imidazolium-tagged glycan probes for non-covalent labeling of live cells

David Benito-alifonso, Shirley Tremell, Joanna C. Sadler, Monica Berry, M. Carmen Galan

Research output: Contribution to journal › Article › peer-review

Abstract

Selective, bioorthogonal and fast labeling of glycoconjugates in living cells is a major challenge for synthetic and cellular biology. Here we report the use imidazolium tagged-mannosamine derivative (ITag-Man) for the non-covalent, rapid and site-specific labeling of sialic acid containing glycoproteins using commercial N-nitrilotriacetate fluorescent reagents in a range of cell lines.

Original language	English
Pages (from-to)	4906-4909
Journal	Chemical Communications
Volume	52
Issue number	27
DOIs	https://doi.org/10.1039/C5CC10040B
Publication status	Published - 7 Apr 2016

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title = "Imidazolium-tagged glycan probes for non-covalent labeling of live cells",

abstract = "Selective, bioorthogonal and fast labeling of glycoconjugates in living cells is a major challenge for synthetic and cellular biology. Here we report the use imidazolium tagged-mannosamine derivative (ITag-Man) for the non-covalent, rapid and site-specific labeling of sialic acid containing glycoproteins using commercial N-nitrilotriacetate fluorescent reagents in a range of cell lines.",

author = "David Benito-alifonso and Shirley Tremell and Sadler, \{Joanna C.\} and Monica Berry and Galan, \{M. Carmen\}",

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AU - Benito-alifonso, David

AU - Tremell, Shirley

AU - Sadler, Joanna C.

AU - Berry, Monica

AU - Galan, M. Carmen

PY - 2016/4/7

Y1 - 2016/4/7

N2 - Selective, bioorthogonal and fast labeling of glycoconjugates in living cells is a major challenge for synthetic and cellular biology. Here we report the use imidazolium tagged-mannosamine derivative (ITag-Man) for the non-covalent, rapid and site-specific labeling of sialic acid containing glycoproteins using commercial N-nitrilotriacetate fluorescent reagents in a range of cell lines.

AB - Selective, bioorthogonal and fast labeling of glycoconjugates in living cells is a major challenge for synthetic and cellular biology. Here we report the use imidazolium tagged-mannosamine derivative (ITag-Man) for the non-covalent, rapid and site-specific labeling of sialic acid containing glycoproteins using commercial N-nitrilotriacetate fluorescent reagents in a range of cell lines.

U2 - 10.1039/C5CC10040B

DO - 10.1039/C5CC10040B

M3 - Article

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VL - 52

SP - 4906

EP - 4909

JO - Chemical Communications

JF - Chemical Communications

IS - 27

ER -

Imidazolium-tagged glycan probes for non-covalent labeling of live cells

Abstract

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