Immediate Remote Ischemic Postconditioning Reduces Brain Nitrotyrosine Formation in a Piglet Asphyxia Model

Eridan Rocha-Ferreira, Brogan Rudge, Michael P Hughes, Ahad A Rahim, Mariya Hristova, Nicola J Robertson

Research output: Contribution to journalArticlepeer-review

Abstract / Description of output

Remote ischemic postconditioning (RIPostC) is a promising therapeutic intervention that could be administered as an alternative to cooling in cases of perinatal hypoxia-ischemia (HI). In the current study we hypothesized that RIPostC in the piglet model of birth asphyxia confers protection by reducing nitrosative stress and subsequent nitrotyrosine formation, as well as having an effect on glial immunoreactivity. Postnatal day 1 (P1) piglets underwent HI brain injury and were randomised to HI (control) or HI + RIPostC. Immunohistochemistry assessment 48 hours after HI revealed a significant decrease in brain nitrotyrosine deposits in the RIPostC-treated group (p = 0.02). This was accompanied by a significant increase in eNOS expression (p < 0.0001) and decrease in iNOS (p = 0.010), with no alteration in nNOS activity. Interestingly, RIPostC treatment was associated with a significant increase in GFAP (p = 0.002) and IBA1 (p = 0.006), markers of astroglial and microglial activity, respectively. The current study demonstrates a beneficial effect of RIPostC therapy in the preclinical piglet model of neonatal asphyxia, which appears to be mediated by modulation of nitrosative stress, despite glial activation.

Original languageEnglish
Pages (from-to)5763743
JournalOxidative Medicine and Cellular Longevity
Volume2016
DOIs
Publication statusPublished - 9 Jun 2016

Keywords / Materials (for Non-textual outputs)

  • Animals
  • Animals, Newborn
  • Asphyxia/metabolism
  • Biomarkers/metabolism
  • Brain/metabolism
  • Disease Models, Animal
  • Female
  • Ischemic Postconditioning
  • Neuroglia/metabolism
  • Nitric Oxide Synthase/metabolism
  • Oxidative Stress
  • Sus scrofa
  • Tyrosine/analogs & derivatives

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