TY - JOUR
T1 - Immobilizing chromate reductase NfoR on magnetic biochar reduced Cr(VI) in copper-containing wastewater
AU - Han, Huawen
AU - Song, Peizhi
AU - Cai, Zhuoshan
AU - Dong, Weijun
AU - Khan, Aman
AU - Yang, Ke
AU - Fang, Yitian
AU - Liu, Pu
AU - Mašek, Ondřej
AU - Li, Xiangkai
N1 - Funding Information:
The authors thank the National Natural Science Foundation Grant (No: 31870082, 32070117) and Fundamental Research Funds for the Central Universities grant (No: lzujbky-2019-cd01, lzujbky-2020-pd06). We would like to thank the Central Laboratory of the School of Life Science, Lanzhou University, for providing various instruments and equipment.
Funding Information:
The authors thank the National Natural Science Foundation Grant (No: 31870082 , 32070117 ) and Fundamental Research Funds for the Central Universities grant (No: lzujbky-2019-cd01 , lzujbky-2020-pd06 ). We would like to thank the Central Laboratory of the School of Life Science, Lanzhou University, for providing various instruments and equipment.
Publisher Copyright:
© 2022 Elsevier Ltd
PY - 2022/5/6
Y1 - 2022/5/6
N2 - Heavy metals (e.g. copper) in smelting wastewater inhibited microbial Cr(VI) reduction, previous report found that a novel chromate reductase NfoR exhibited Cu(II)-enhanced activity and broad-spectrum metal tolerance. In this study, magnetic biochar (MB) from pine sawdust was fabricated to immobilize NfoR via covalent binding. MB-NfoR showed a 2.6- and 2.1-folds higher activity in reducing Cr(VI) to Cr(III) compared to free NfoR and MB, respectively. XPS and EPR analysis showed that NfoR catalysis and persistent free radicals (PFRs) are responsible for Cr(VI) reduction mechanism of MB-NfoR. The addition of Cu(II) increased the MB-NfoR activity 2.3-fold compared to that of without Cu(II). The maximum Cr(VI) removal by MB-NfoR in the pure Cr(VI) solution was 98% at 45 °C and pH 6.0, and the MB-NfoR retained 68.3% of its initial activity even after five consecutive cycles. Application of MB-NfoR in smelting wastewater can simultaneously remove 94% Cr(VI) and 52.1% Cu(II). Furthermore, treated smelting wastewater by MB-NfoR showed no phytotoxicity and enriched numerous oligotrophic bacteria. This study provides a novel ‘one-stop’ strategy for decontamination of effluents combined with heavy metals, utilizing synergies offered by constituents in such waste streams.
AB - Heavy metals (e.g. copper) in smelting wastewater inhibited microbial Cr(VI) reduction, previous report found that a novel chromate reductase NfoR exhibited Cu(II)-enhanced activity and broad-spectrum metal tolerance. In this study, magnetic biochar (MB) from pine sawdust was fabricated to immobilize NfoR via covalent binding. MB-NfoR showed a 2.6- and 2.1-folds higher activity in reducing Cr(VI) to Cr(III) compared to free NfoR and MB, respectively. XPS and EPR analysis showed that NfoR catalysis and persistent free radicals (PFRs) are responsible for Cr(VI) reduction mechanism of MB-NfoR. The addition of Cu(II) increased the MB-NfoR activity 2.3-fold compared to that of without Cu(II). The maximum Cr(VI) removal by MB-NfoR in the pure Cr(VI) solution was 98% at 45 °C and pH 6.0, and the MB-NfoR retained 68.3% of its initial activity even after five consecutive cycles. Application of MB-NfoR in smelting wastewater can simultaneously remove 94% Cr(VI) and 52.1% Cu(II). Furthermore, treated smelting wastewater by MB-NfoR showed no phytotoxicity and enriched numerous oligotrophic bacteria. This study provides a novel ‘one-stop’ strategy for decontamination of effluents combined with heavy metals, utilizing synergies offered by constituents in such waste streams.
U2 - 10.1016/j.jclepro.2022.132118
DO - 10.1016/j.jclepro.2022.132118
M3 - Article
SN - 0959-6526
JO - Journal of Cleaner Production
JF - Journal of Cleaner Production
M1 - 132118
ER -