Abstract
Nine different adjuvants were examined for their ability to potentiate the humoral and cell-mediated immune responses of cattle to a soluble glycoprotein antigen prepared from Trypanosoma brucei. Serological responses as measured by the Farr assay were best augmented by the oil-based adjuvants and saponin. Cell-mediated immunity, as assessed by specific lymphocyte transformation in vitro, was enhanced by all oil-based adjuvants at different intervals after immunization. Results from a challenge infection of immunized cattle with the homologous clone of T. brucei and from neutralization tests indicated that protection against infection was better correlated with specific antibodies than with cell-mediated responses. From these considerations, and the absence of tissue reactions at the site of inoculation, saponin was considered more practical than the oil-based or bacterial adjuvants for the elicitation in cattle of antibodies to purified soluble antigens.
Original language | English |
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Pages (from-to) | 1-10 |
Number of pages | 10 |
Journal | Infection and Immunity |
Volume | 36 |
Issue number | 1 |
Publication status | Published - Apr 1982 |
Keywords / Materials (for Non-textual outputs)
- Acetylmuramyl-Alanyl-Isoglutamine/immunology
- Adjuvants, Immunologic/pharmacology
- Animals
- Antibody Formation
- Antigens, Surface/immunology
- Bordetella pertussis/immunology
- Cattle/immunology
- Freund's Adjuvant/pharmacology
- Immunization/veterinary
- Lymphocyte Activation
- Poly A-U/immunology
- Propionibacterium acnes/immunology
- Trypanosoma brucei brucei/immunology
- Trypanosomiasis, Bovine/immunology
- Trypanosomiasis, Bovine/prevention & control