Immunization of cattle with a variant-specific surface antigen of Trypanosoma brucei: influence of different adjuvants

P W Wells, D.L. Emery, C A Hinson, Ivan Morrison, M. Murray

Research output: Contribution to journalArticlepeer-review


Nine different adjuvants were examined for their ability to potentiate the humoral and cell-mediated immune responses of cattle to a soluble glycoprotein antigen prepared from Trypanosoma brucei. Serological responses as measured by the Farr assay were best augmented by the oil-based adjuvants and saponin. Cell-mediated immunity, as assessed by specific lymphocyte transformation in vitro, was enhanced by all oil-based adjuvants at different intervals after immunization. Results from a challenge infection of immunized cattle with the homologous clone of T. brucei and from neutralization tests indicated that protection against infection was better correlated with specific antibodies than with cell-mediated responses. From these considerations, and the absence of tissue reactions at the site of inoculation, saponin was considered more practical than the oil-based or bacterial adjuvants for the elicitation in cattle of antibodies to purified soluble antigens.
Original languageEnglish
Pages (from-to)1-10
Number of pages10
JournalInfection and Immunity
Issue number1
Publication statusPublished - Apr 1982


  • Acetylmuramyl-Alanyl-Isoglutamine/immunology
  • Adjuvants, Immunologic/pharmacology
  • Animals
  • Antibody Formation
  • Antigens, Surface/immunology
  • Bordetella pertussis/immunology
  • Cattle/immunology
  • Freund's Adjuvant/pharmacology
  • Immunization/veterinary
  • Lymphocyte Activation
  • Poly A-U/immunology
  • Propionibacterium acnes/immunology
  • Trypanosoma brucei brucei/immunology
  • Trypanosomiasis, Bovine/immunology
  • Trypanosomiasis, Bovine/prevention & control


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