Immunocytochemical techniques to investigate the pathogenesis of infectious micro-organisms and the concurrent immune response of the host

SHM Jeurissen*, E Claassen, AG Boonstra-Blom, L Vervelde, EM Janse

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

For poultry as well as for mammalian species used for scientific research, many immunocytochemical techniques have been developed to investigate in detail the interaction between infectious micro-organisms and the nonspecific and specific immune systems of the host. In this review three techniques have been described with all technical details necessary to perform them correctly: (1) single immunocytochemical staining to detect the infectious microorganisms in situ at their site of infection, (2) double immunocytochemical staining to visualize simultaneously the infectious micro-organism and the host cellular response to investigate their interactions, and (3) detection of plasma cells producing antibodies specific to the micro-organism. Of the three techniques the results are described when applied on chicken tissues infected with various micro-organisms, such as Marek's disease virus, chicken anemia virus, infectious bursal disease virus and Eimeria tenella. (C) 2000 Elsevier Science Ltd. All rights reserved.

Original languageEnglish
Pages (from-to)141-151
Number of pages11
JournalDevelopmental and Comparative Immunology
Volume24
Issue number2-3
DOIs
Publication statusPublished - 2000

Keywords

  • LEUKOCYTES
  • horseradish peroxidase
  • in situ detection
  • IN-SITU
  • alkaline phosphatase
  • CELLS
  • immunocytochemical staining
  • BURSAL DISEASE
  • chicken
  • beta-galactosidase
  • COMPLEX
  • CHICKENS
  • MONOCLONAL-ANTIBODIES
  • VIRUS
  • monoclonal antibody
  • EIMERIA-TENELLA

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