Immunohistochemical Expression of CCR2, CSF1R and MMP9 in Canine Inflammatory Mammary Carcinomas

Teresa Raposo, Breno C B Beirão, Isabel Pires, Justina Prada, Paula Brilhante, David Argyle, Felisbina L. Queiroga

Research output: Contribution to journalArticlepeer-review

Abstract

Background: Canine inflammatory mammary cancer (IMC) and its human counterpart, inflammatory breast cancer, are extremely aggressive types of cancer. Our aim was to characterize immunohistochemical expression of C-C chemokine receptor 2, colony stimulating factor 1 receptor and metalloproteinase-9 in canine IMC versus non-IMC and to analyze associations with clinicopathological variables.

Materials and Methods: Immunohistochemical staining of CCR2, CSF1R and MMP9 was performed in series of 25 IMC and 15 non-IMC tumors.

Results: No differences in the expression of these biomarkers between IMC and non-IMC were observed. Distinct nuclear subcellular expression of CCR2 was observed in IMC (p<0.001). For IMC, higher CCR2 expression was associated with increased nuclear grade (p=0.037), and higher neoplastic MMP9 expression was associated with fewer mitoses (p=0.022), higher nuclear grade (p=0.047) and increased CSF1R expression (p=0.025).

Conclusion: Expression of CCR2, CSF1R and MMP9 in canine IMC could contribute to increased nuclear pleomorphism but the biological mechanisms involved warrant further investigation.
Original languageEnglish
Pages (from-to)1805-13
Number of pages9
JournalAnticancer research
Volume36
Issue number4
Early online date14 Apr 2016
Publication statusE-pub ahead of print - 14 Apr 2016

Keywords

  • CCR2
  • CSF1R
  • Canine inflammatory mammary carcinomas
  • MMP9
  • canine mammary tumours
  • tumour microenvironment

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