Immunohistochemical localization of a macrophage-specific antigen in developing mouse retina: phagocytosis of dying neurons and differentiation of microglial cells to form a regular array in the plexiform layers

D A Hume, V H Perry, S Gordon

Research output: Contribution to journalArticlepeer-review

Abstract

In the developing mouse retina degenerating neurons can be observed initially in the ganglion cell layer followed by a phase of cell death in the inner nuclear layer. Using an immunohistochemical method to localize the mouse macrophage specific antigen F4/80, we show that macrophages migrate from the vascular supply overlying the developing retina and phagocytose the degenerating neurons. The macrophages subsequently differentiate to become the microglia of the retina and form a regularly spaced distribution across the retina in the inner and outer plexiform layers. These experiments provide strong evidence for the mesodermal origin of central nervous system microglia.
Original languageEnglish
Pages (from-to)253-7
Number of pages5
JournalJournal of Cell Biology
Volume97
Issue number1
Publication statusPublished - Jul 1983

Keywords

  • Animals
  • Antigens
  • Cell Differentiation
  • Cell Survival
  • Immunoenzyme Techniques
  • Macrophages
  • Mice
  • Mice, Inbred BALB C
  • Mice, Inbred C57BL
  • Neuroglia
  • Neurons
  • Phagocytosis
  • Retina

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