Immunohistochemical localization of prion protein in spongiform encephalopathies and normal brain tissue

P Piccardo, J Safar, M Ceroni, D C Gajdusek, C J Gibbs

Research output: Contribution to journalArticlepeer-review

Abstract / Description of output

We used polyclonal antibodies raised against hamster and mouse PrP27-30 as immunologic probes to study the localization of intracellular and extracellular deposits of prion protein in normal and scrapie-infected mouse and hamster brains and in Creutzfeldt-Jakob disease (CJD)-infected mouse brains. In addition, we examined normal human brain and brain tissues from patients with CJD, kuru, Alzheimer's disease, and idiopathic chronic encephalitis. There was positive staining in the cytoplasm of neurons of normal and scrapie- and CJD-infected mice, and in the neurons of normal and scrapie-infected hamsters. The staining pattern suggests the localization of PrP in an intracellular membrane compartment, most likely the rough endoplasmic reticulum or Golgi apparatus. Antibodies raised against a 15-amino-acid synthetic peptide of the N-terminal of hamster PrP27-30 displayed a similar pattern of staining in mouse brain sections. We observed no intracellular staining in human brain sections obtained at autopsy. Antibodies prepared against mouse and hamster PrP27-30 reacted with amyloid plaques in scrapie-infected mouse and kuru- and CJD-infected human brain sections but not with amyloid plaques in the brain of a patient with Alzheimer's disease.

Original languageEnglish
Pages (from-to)518-22
Number of pages5
Issue number3 Pt 1
Publication statusPublished - Mar 1990

Keywords / Materials (for Non-textual outputs)

  • Alzheimer Disease
  • Amyloid
  • Animals
  • Brain Chemistry
  • Creutzfeldt-Jakob Syndrome
  • Cricetinae
  • Humans
  • Immunohistochemistry
  • Kuru
  • Mice
  • PrP 27-30 Protein
  • Prions
  • Scrapie
  • Staining and Labeling
  • Viral Proteins


Dive into the research topics of 'Immunohistochemical localization of prion protein in spongiform encephalopathies and normal brain tissue'. Together they form a unique fingerprint.

Cite this