Immunohistological analysis of the immunoreactivity of normal lymphoid cells and lymphomas with the monoclonal antibody OPD4

J O'Grady, A J Blaikie, S E Howie, D M Salter, A S Krajewski

Research output: Contribution to journalArticlepeer-review

Abstract

OPD4 is a recently described monoclonal antibody that recognizes a fixation-resistant 200 kD antigen restricted to a subset of T cells. Immunolabelling with OPD4 in paraffin sections of normal lymphoid tissues and cases of malignant lymphoma was compared with that of other antibodies in common use, including the T-cell restricted antibodies MT1 and UCHL1 and the B-cell restricted antibodies MB1, F8-11-13, and L26. OPD4 showed similar immunoreactivity to UCHL1 in normal tissues. OPD4 did not stain Reed-Sternberg cells in Hodgkin's disease. In non-Hodgkin's lymphomas, OPD4, like UCHL1, reacted with only 2/22 B-cell lymphomas. OPD4 was, however, less useful as a marker of T-cell lymphomas, staining only 11/32 cases, while UCHL1 stained 22/32 cases. We conclude that OPD4 is not a useful antibody for the routine diagnosis of T-cell lymphoma.

Original languageEnglish
Pages (from-to)119-25
Number of pages7
JournalThe Journal of Pathology
Volume164
Issue number2
DOIs
Publication statusPublished - Jun 1991

Keywords

  • Adult
  • Antibodies, Monoclonal
  • Child
  • Hodgkin Disease
  • Humans
  • Immunoenzyme Techniques
  • Lymph Nodes
  • Lymphoid Tissue
  • Lymphoma
  • Lymphoma, B-Cell
  • Lymphoma, T-Cell
  • Spleen
  • Thymus Gland

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