Immunological evidence that HMG-CoA reductase kinase-A is the cauliflower homologue of the RKIN1 subfamily of plant protein kinases

K L Ball; J Barker; N G Halford; D G Hardie

doi:10.1016/0014-5793(95)01343-1

Immunological evidence that HMG-CoA reductase kinase-A is the cauliflower homologue of the RKIN1 subfamily of plant protein kinases

K L Ball, J Barker, N G Halford, D G Hardie

Research output: Contribution to journal › Article › peer-review

Abstract / Description of output

Three different antibodies against the RKIN1 and BKIN12 gene products from rye and barley recognized the 58 kDa subunit of HMG-CoA reductase kinase-A (HRK-A) from Brassica oleracea on Western blots. HRK-A was also detected by an antipeptide antibody in enzyme-linked immunoassays, and this was competed by the peptide antigen. HRK-A was not recognized by antibodies against plant, mammalian and Saccharomyces cerevisiae relatives of RKIN1, i.e. wheat PKABA1, rat AMP-activated protein kinase and S. cerevisiae Snf1p. RKIN1/HMG-CoA reductase kinase-A are now among the first protein kinases in plants to be well characterized at both the molecular and biochemical levels.

Original language	English
Pages (from-to)	189-92
Number of pages	4
Journal	FEBS Letters
Volume	377
Issue number	2
DOIs	https://doi.org/10.1016/0014-5793(95)01343-1
Publication status	Published - 18 Dec 1995

Keywords / Materials (for Non-textual outputs)

AMP-Activated Protein Kinases
Amino Acid Sequence
Animals
Antibodies
Brassica
Cross Reactions
Fungal Proteins
Molecular Sequence Data
Multienzyme Complexes
Peptides
Plant Proteins
Protein Kinases
Protein-Serine-Threonine Kinases
Rats

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10.1016/0014-5793(95)01343-1

Immunological evidence that HMG-CoA reductase kinase-A is the cauliflower homologue of the RKIN1 subfamily of plant protein kinases
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http://www.sciencedirect.com/science/article/pii/0014579395013431

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title = "Immunological evidence that HMG-CoA reductase kinase-A is the cauliflower homologue of the RKIN1 subfamily of plant protein kinases",

abstract = "Three different antibodies against the RKIN1 and BKIN12 gene products from rye and barley recognized the 58 kDa subunit of HMG-CoA reductase kinase-A (HRK-A) from Brassica oleracea on Western blots. HRK-A was also detected by an antipeptide antibody in enzyme-linked immunoassays, and this was competed by the peptide antigen. HRK-A was not recognized by antibodies against plant, mammalian and Saccharomyces cerevisiae relatives of RKIN1, i.e. wheat PKABA1, rat AMP-activated protein kinase and S. cerevisiae Snf1p. RKIN1/HMG-CoA reductase kinase-A are now among the first protein kinases in plants to be well characterized at both the molecular and biochemical levels.",

keywords = "AMP-Activated Protein Kinases, Amino Acid Sequence, Animals, Antibodies, Brassica, Cross Reactions, Fungal Proteins, Molecular Sequence Data, Multienzyme Complexes, Peptides, Plant Proteins, Protein Kinases, Protein-Serine-Threonine Kinases, Rats",

author = "Ball, {K L} and J Barker and Halford, {N G} and Hardie, {D G}",

year = "1995",

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doi = "10.1016/0014-5793(95)01343-1",

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T1 - Immunological evidence that HMG-CoA reductase kinase-A is the cauliflower homologue of the RKIN1 subfamily of plant protein kinases

AU - Ball, K L

AU - Barker, J

AU - Halford, N G

AU - Hardie, D G

PY - 1995/12/18

Y1 - 1995/12/18

N2 - Three different antibodies against the RKIN1 and BKIN12 gene products from rye and barley recognized the 58 kDa subunit of HMG-CoA reductase kinase-A (HRK-A) from Brassica oleracea on Western blots. HRK-A was also detected by an antipeptide antibody in enzyme-linked immunoassays, and this was competed by the peptide antigen. HRK-A was not recognized by antibodies against plant, mammalian and Saccharomyces cerevisiae relatives of RKIN1, i.e. wheat PKABA1, rat AMP-activated protein kinase and S. cerevisiae Snf1p. RKIN1/HMG-CoA reductase kinase-A are now among the first protein kinases in plants to be well characterized at both the molecular and biochemical levels.

AB - Three different antibodies against the RKIN1 and BKIN12 gene products from rye and barley recognized the 58 kDa subunit of HMG-CoA reductase kinase-A (HRK-A) from Brassica oleracea on Western blots. HRK-A was also detected by an antipeptide antibody in enzyme-linked immunoassays, and this was competed by the peptide antigen. HRK-A was not recognized by antibodies against plant, mammalian and Saccharomyces cerevisiae relatives of RKIN1, i.e. wheat PKABA1, rat AMP-activated protein kinase and S. cerevisiae Snf1p. RKIN1/HMG-CoA reductase kinase-A are now among the first protein kinases in plants to be well characterized at both the molecular and biochemical levels.

KW - AMP-Activated Protein Kinases

KW - Amino Acid Sequence

KW - Animals

KW - Antibodies

KW - Brassica

KW - Cross Reactions

KW - Fungal Proteins

KW - Molecular Sequence Data

KW - Multienzyme Complexes

KW - Peptides

KW - Plant Proteins

KW - Protein Kinases

KW - Protein-Serine-Threonine Kinases

KW - Rats

U2 - 10.1016/0014-5793(95)01343-1

DO - 10.1016/0014-5793(95)01343-1

M3 - Article

C2 - 8543048

SN - 0014-5793

VL - 377

SP - 189

EP - 192

JO - FEBS Letters

JF - FEBS Letters

IS - 2

ER -

Immunological evidence that HMG-CoA reductase kinase-A is the cauliflower homologue of the RKIN1 subfamily of plant protein kinases

Abstract / Description of output

Keywords / Materials (for Non-textual outputs)

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