Immunological evidence that HMG-CoA reductase kinase-A is the cauliflower homologue of the RKIN1 subfamily of plant protein kinases

K L Ball, J Barker, N G Halford, D G Hardie

Research output: Contribution to journalArticlepeer-review

Abstract

Three different antibodies against the RKIN1 and BKIN12 gene products from rye and barley recognized the 58 kDa subunit of HMG-CoA reductase kinase-A (HRK-A) from Brassica oleracea on Western blots. HRK-A was also detected by an antipeptide antibody in enzyme-linked immunoassays, and this was competed by the peptide antigen. HRK-A was not recognized by antibodies against plant, mammalian and Saccharomyces cerevisiae relatives of RKIN1, i.e. wheat PKABA1, rat AMP-activated protein kinase and S. cerevisiae Snf1p. RKIN1/HMG-CoA reductase kinase-A are now among the first protein kinases in plants to be well characterized at both the molecular and biochemical levels.
Original languageEnglish
Pages (from-to)189-92
Number of pages4
JournalFEBS Letters
Volume377
Issue number2
DOIs
Publication statusPublished - 18 Dec 1995

Keywords

  • AMP-Activated Protein Kinases
  • Amino Acid Sequence
  • Animals
  • Antibodies
  • Brassica
  • Cross Reactions
  • Fungal Proteins
  • Molecular Sequence Data
  • Multienzyme Complexes
  • Peptides
  • Plant Proteins
  • Protein Kinases
  • Protein-Serine-Threonine Kinases
  • Rats

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