Impact of alternative initiation, splicing, and termination on the diversity of the mRNA transcripts encoded by the mouse transcriptome

RIKEN GER Group, Mihaela Zavolan, Shinji Kondo, Christian Schonbach, Jun Adachi, David A Hume, Yoshihide Hayashizaki, Terry Gaasterland

Research output: Contribution to journalArticlepeer-review


We analyzed the FANTOM2 clone set of 60,770 RIKEN full-length mouse cDNA sequences and 44,122 public mRNA sequences. We developed a new computational procedure to identify and classify the forms of splice variation evident in this data set and organized the results into a publicly accessible database that can be used for future expression array construction, structural genomics, and analyses of the mechanism and regulation of alternative splicing. Statistical analysis shows that at least 41% and possibly as much as 60% of multiexon genes in mouse have multiple splice forms. Of the transcription units with multiple splice forms, 49% contain transcripts in which the apparent use of an alternative transcription start (stop) is accompanied by alternative splicing of the initial (terminal) exon. This implies that alternative transcription may frequently induce alternative splicing. The fact that 73% of all exons with splice variation fall within the annotated coding region indicates that most splice variation is likely to affect the protein form. Finally, we compared the set of constitutive (present in all transcripts) exons with the set of cryptic (present only in some transcripts) exons and found statistically significant differences in their length distributions, the nucleotide distributions around their splice junctions, and the frequencies of occurrence of several short sequence motifs.
Original languageEnglish
Pages (from-to)1290-300
Number of pages11
JournalGenome Research
Issue number6B
Publication statusPublished - Jun 2003


  • Alternative Splicing
  • Animals
  • Cluster Analysis
  • Computational Biology
  • Databases, Genetic
  • Exons
  • Genes
  • Genetic Variation
  • Genome
  • Mice
  • Polypyrimidine Tract-Binding Protein
  • Proteome
  • RNA Splice Sites
  • RNA Splicing
  • RNA, Messenger
  • Transcription Initiation Site
  • Transcription, Genetic


Dive into the research topics of 'Impact of alternative initiation, splicing, and termination on the diversity of the mRNA transcripts encoded by the mouse transcriptome'. Together they form a unique fingerprint.

Cite this