Impact of microfluidic processing on bacterial ribonucleic acid expression

Senthil Kumar Gandi, David Watson, Maiwenn Kersaudy-Kerhoas, Marc P Y Desmulliez, Till Bachmann, Helen Bridle

Research output: Contribution to journalArticlepeer-review

Abstract / Description of output

Bacterial transcriptomics is widely used to investigate gene regulation, bacterial susceptibility to antibiotics, host-pathogen interactions, and pathogenesis. Transcriptomics is crucially dependent on suitable methods to isolate and detect bacterial RNA. Microfluidics offer ways of creating integrated point-of-care systems, analysing a sample from preparation, and RNA isolation to detection. A critical requirement for on-chip diagnostics to deliver on their promise is that mRNA expression is not altered via microfluidic sample processing. This article investigates the impact of the use of microfluidics upon RNA expression of bacteria isolated from blood, a key step towards proving the suitability of such systems for further development.

Original languageEnglish
Pages (from-to)031102
Issue number3
Publication statusPublished - May 2015


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