Impaired gluconeogenesis in a porcine model of paracetamol induced acute liver failure

Konstantinos J. Dabos; Henry R. Whalen; Philip N. Newsome; John A. Parkinson; Neil C. Henderson; Ian H. Sadler; Peter C. Hayes; John Plevris

doi:10.3748/wjg.v17.i11.1457

Impaired gluconeogenesis in a porcine model of paracetamol induced acute liver failure

Konstantinos J. Dabos, Henry R. Whalen, Philip N. Newsome, John A. Parkinson, Neil C. Henderson, Ian H. Sadler, Peter C. Hayes, John Plevris

Research output: Contribution to journal › Article › peer-review

Abstract

AIM: To investigate glucose homeostasis and in particular gluconeogenesis in a large animal model of acute liver failure (ALF).

METHODS: Six pigs with paracetamol induced ALF under general anaesthesia were studied over 25 h. Plasma samples were withdrawn every five hours from a central vein. Three animals were used as controls and were maintained under anaesthesia only. Using H-1 NMR spectroscopy we identified most gluconeogenic amino acids along with lactate and pyruvate in the animal plasma samples.

RESULTS: No significant changes were observed in the concentrations of the amino acids studied in the animals maintained under anaesthesia only. If we look at the ALF animals, we observed a statistically significant rise of lactate (P < 0.003) and pyruvate (P < 0.018) at the end of the experiments. We also observed statistically significant rises in the concentrations of alanine (P < 0.002), glycine (P < 0.005), threonine (P < 0.048), tyrosine (P < 0.000), phenylalanine (P < 0.000) and isoleucine (P < 0.01). Valine levels decreased significantly (P < 0.05).

Original language	English
Pages (from-to)	1457-1461
Number of pages	5
Journal	World Journal of Gastroenterology
Volume	17
Issue number	11
DOIs	https://doi.org/10.3748/wjg.v17.i11.1457
Publication status	Published - 21 Mar 2011

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@article{e5338f1ef48b4556ae4e237c0a4f60f0,

title = "Impaired gluconeogenesis in a porcine model of paracetamol induced acute liver failure",

abstract = "AIM: To investigate glucose homeostasis and in particular gluconeogenesis in a large animal model of acute liver failure (ALF).METHODS: Six pigs with paracetamol induced ALF under general anaesthesia were studied over 25 h. Plasma samples were withdrawn every five hours from a central vein. Three animals were used as controls and were maintained under anaesthesia only. Using H-1 NMR spectroscopy we identified most gluconeogenic amino acids along with lactate and pyruvate in the animal plasma samples.RESULTS: No significant changes were observed in the concentrations of the amino acids studied in the animals maintained under anaesthesia only. If we look at the ALF animals, we observed a statistically significant rise of lactate (P < 0.003) and pyruvate (P < 0.018) at the end of the experiments. We also observed statistically significant rises in the concentrations of alanine (P < 0.002), glycine (P < 0.005), threonine (P < 0.048), tyrosine (P < 0.000), phenylalanine (P < 0.000) and isoleucine (P < 0.01). Valine levels decreased significantly (P < 0.05).CONCLUSION: Our pig model of ALF is characterized by an altered gluconeogenetic capacity, an impaired tricarboxylic acid (TCA) cycle and a glycolytic state. (C) 2011 Baishideng. All rights reserved.",

author = "Dabos, {Konstantinos J.} and Whalen, {Henry R.} and Newsome, {Philip N.} and Parkinson, {John A.} and Henderson, {Neil C.} and Sadler, {Ian H.} and Hayes, {Peter C.} and John Plevris",

year = "2011",

month = mar,

day = "21",

doi = "10.3748/wjg.v17.i11.1457",

language = "English",

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pages = "1457--1461",

journal = "World Journal of Gastroenterology",

issn = "1007-9327",

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Impaired gluconeogenesis in a porcine model of paracetamol induced acute liver failure. / Dabos, Konstantinos J.; Whalen, Henry R.; Newsome, Philip N.; Parkinson, John A.; Henderson, Neil C.; Sadler, Ian H.; Hayes, Peter C.; Plevris, John.

In: World Journal of Gastroenterology, Vol. 17, No. 11, 21.03.2011, p. 1457-1461.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Impaired gluconeogenesis in a porcine model of paracetamol induced acute liver failure

AU - Dabos, Konstantinos J.

AU - Whalen, Henry R.

AU - Newsome, Philip N.

AU - Parkinson, John A.

AU - Henderson, Neil C.

AU - Sadler, Ian H.

AU - Hayes, Peter C.

AU - Plevris, John

PY - 2011/3/21

Y1 - 2011/3/21

N2 - AIM: To investigate glucose homeostasis and in particular gluconeogenesis in a large animal model of acute liver failure (ALF).METHODS: Six pigs with paracetamol induced ALF under general anaesthesia were studied over 25 h. Plasma samples were withdrawn every five hours from a central vein. Three animals were used as controls and were maintained under anaesthesia only. Using H-1 NMR spectroscopy we identified most gluconeogenic amino acids along with lactate and pyruvate in the animal plasma samples.RESULTS: No significant changes were observed in the concentrations of the amino acids studied in the animals maintained under anaesthesia only. If we look at the ALF animals, we observed a statistically significant rise of lactate (P < 0.003) and pyruvate (P < 0.018) at the end of the experiments. We also observed statistically significant rises in the concentrations of alanine (P < 0.002), glycine (P < 0.005), threonine (P < 0.048), tyrosine (P < 0.000), phenylalanine (P < 0.000) and isoleucine (P < 0.01). Valine levels decreased significantly (P < 0.05).CONCLUSION: Our pig model of ALF is characterized by an altered gluconeogenetic capacity, an impaired tricarboxylic acid (TCA) cycle and a glycolytic state. (C) 2011 Baishideng. All rights reserved.

AB - AIM: To investigate glucose homeostasis and in particular gluconeogenesis in a large animal model of acute liver failure (ALF).METHODS: Six pigs with paracetamol induced ALF under general anaesthesia were studied over 25 h. Plasma samples were withdrawn every five hours from a central vein. Three animals were used as controls and were maintained under anaesthesia only. Using H-1 NMR spectroscopy we identified most gluconeogenic amino acids along with lactate and pyruvate in the animal plasma samples.RESULTS: No significant changes were observed in the concentrations of the amino acids studied in the animals maintained under anaesthesia only. If we look at the ALF animals, we observed a statistically significant rise of lactate (P < 0.003) and pyruvate (P < 0.018) at the end of the experiments. We also observed statistically significant rises in the concentrations of alanine (P < 0.002), glycine (P < 0.005), threonine (P < 0.048), tyrosine (P < 0.000), phenylalanine (P < 0.000) and isoleucine (P < 0.01). Valine levels decreased significantly (P < 0.05).CONCLUSION: Our pig model of ALF is characterized by an altered gluconeogenetic capacity, an impaired tricarboxylic acid (TCA) cycle and a glycolytic state. (C) 2011 Baishideng. All rights reserved.

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JO - World Journal of Gastroenterology

JF - World Journal of Gastroenterology

SN - 1007-9327

IS - 11

ER -

Impaired gluconeogenesis in a porcine model of paracetamol induced acute liver failure

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