@inbook{8834b27b32b645888ae2ed82e98f8528,
title = "Improved denaturation of small RNA duplexes and it's application for northern blotting",
abstract = "Small RNAs (sRNAs) are short (18-30 nucleotide) noncoding RNA molecules, which control gene expression and pathogen response in eukaryotes. They are associated with and guide nucleases to target nucleic acids by nucleotide base pairing. We found that current techniques for small RNA detection are adversely affected by the presence of complementary RNA. Thus we established FDF-PAGE (fully denaturing formaldehyde polyacrylamide gel electrophoresis), which dramatically improves denaturation efficiency and subsequently the detection of sequestered sRNAs.",
keywords = "small RNA, sequestration, polyacrylamide gel electrophoresis, denaturation, improved detection",
author = "Harris, {C. Jake} and Baulcombe, {David C.} and Attila Molnar",
year = "2017",
month = apr,
day = "25",
doi = "10.1007/978-1-4939-6866-4_1",
language = "English",
isbn = "978-1-4939-6864-0",
series = "Methods in Molecular Biology",
publisher = "HUMANA PRESS INC",
pages = "1--6",
editor = "T Dalmay",
booktitle = "Microrna detection and target identification",
}