Improved denaturation of small RNA duplexes and it's application for northern blotting

C. Jake Harris*, David C. Baulcombe, Attila Molnar

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Small RNAs (sRNAs) are short (18-30 nucleotide) noncoding RNA molecules, which control gene expression and pathogen response in eukaryotes. They are associated with and guide nucleases to target nucleic acids by nucleotide base pairing. We found that current techniques for small RNA detection are adversely affected by the presence of complementary RNA. Thus we established FDF-PAGE (fully denaturing formaldehyde polyacrylamide gel electrophoresis), which dramatically improves denaturation efficiency and subsequently the detection of sequestered sRNAs.
Original languageEnglish
Title of host publicationMicrorna detection and target identification
Subtitle of host publicationMethods and protocols
EditorsT Dalmay
PublisherHUMANA PRESS INC
Pages1-6
Number of pages6
ISBN (Electronic)978-1-4939-6866-4
ISBN (Print)978-1-4939-6864-0
DOIs
Publication statusE-pub ahead of print - 25 Apr 2017

Publication series

NameMethods in Molecular Biology
PublisherHUMANA PRESS INC
Volume1580
ISSN (Print)1064-3745

Keywords

  • small RNA
  • sequestration
  • polyacrylamide gel electrophoresis
  • denaturation
  • improved detection

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