Improved peptide backbone fragmentation is the primary advantage of MS-cleavable crosslinkers

Lars Kolbowski, Swantje Lenz, Lutz Fischer, Ludwig Sinn, Francis J. O'Reilly, Juri Rappsilber

Research output: Contribution to journalArticlepeer-review


Proteome-wide crosslinking mass spectrometry studies have coincided with the advent of MS-cleavable crosslinkers that can reveal the individual masses of the two crosslinked peptides. However, recently such studies have also been published with non-cleavable crosslinkers suggesting that MS-cleavability is not essential. We therefore examined in detail the advantages and disadvantages of using the commonly used MS-cleavable crosslinker, DSSO. Indeed, DSSO gave rise to signature peptide fragments with a distinct mass difference (doublet) for nearly all identified crosslinked peptides. Surprisingly, we could show that it was not these peptide masses that proved the main advantage of MS-cleavability of the crosslinker, but improved peptide backbone fragmentation which reduces ambiguity of peptide identifications. This also holds true for another commonly used MS-cleavable crosslinker, DSBU. We show furthermore, that the more intricate MS3-based data acquisition approaches lack sensitivity and specificity, causing them to be outperformed by the simpler and faster stepped HCD method. This understanding will guide future developments and applications of proteome-wide crosslinking mass spectrometry.
Original languageEnglish
Pages (from-to)7779-7786
Number of pages8
JournalAnalytical Chemistry
Publication statusPublished - 25 May 2022


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