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Abstract
Snapshot imaging has a number of advantages in automated gel electrophoresis compared with the finish-line method in capillary electrophoresis, although at the expense of resolution. This paper presents a novel signal processing algorithm enabling a multi-capture imaging modality which improves resolution. The approach takes multiple snapshots as macromolecules are electrophroresed. Peaks from latter snapshots have higher resolution but poor signal-to-noise ratio (SNR), while peaks from earlier snapshots have lower resolution but better SNR. Signals at different capture-times are related by a scale-in-separation, amplitude scaling, and an arbitrary shift. The multiple captures are realigned and fused together using least-squares estimation and a physically inspired signal model. Since partial waveforms are observed as the chromatic peaks exit the sensor's field-of-view, this is accounted for in the realignment algorithm. The proposed technique yields improved resolution, improved fragment concentration and size estimates, and allows the removal of static background noise.
Original language | English |
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Title of host publication | European Signal Processing Conference (EUSIPCO) |
DOIs | |
Publication status | Published - 28 Aug 2016 |
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Dive into the research topics of 'Improved Resolution of Chromatographic Peak Analysis using Multi-Snapshot Imaging'. Together they form a unique fingerprint.Projects
- 1 Finished
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Novel Signal Processing Algorithms for Automated Electrophoresis Analysis
Hopgood, J. (Principal Investigator)
1/09/15 → 31/08/16
Project: Research
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Multi-Snapshot Imaging for Chromatographic Peak Analysis
Hopgood, J., Connelly, M., McHoull, B. & Troy, D., 12 Apr 2018, In: IEEE Transactions on Biomedical Engineering. 66, 1, p. 119-129Research output: Contribution to journal › Article › peer-review
Open AccessFile -
A New Approach for Multi-Dimensional Signal Processing and Modelling for Signals From Gel Electrophoresis
Yousif, E. H. G., Hopgood, J., Thompson, J. & Davies, M., 28 May 2016, European Signal Processing Conference (EUSIPCO).Research output: Chapter in Book/Report/Conference proceeding › Conference contribution