Improving cartilage phenotype from differentiated pericytes in tunable peptide hydrogels

Enateri V Alakpa, Vineetha Jayawarna, Karl E V Burgess, Christopher C West, Bruno Péault, Rein V Ulijn, Matthew J Dalby

Research output: Contribution to journalArticlepeer-review


Differentiation of stem cells to chondrocytes in vitro usually results in a heterogeneous phenotype. This is evident in the often detected over expression of type X collagen which, in hyaline cartilage structure is not characteristic of the mid-zone but of the deep-zone ossifying tissue. Methods to better match cartilage developed in vitro to characteristic in vivo features are therefore highly desirable in regenerative medicine. This study compares phenotype characteristics between pericytes, obtained from human adipose tissue, differentiated using diphenylalanine/serine (F2/S) peptide hydrogels with the more widely used chemical induced method for chondrogenesis. Significantly higher levels of type II collagen were noted when pericytes undergo chondrogenesis in the hydrogel in the absence of induction media. There is also a balanced expression of collagen relative to aggrecan production, a feature which was biased toward collagen production when cells were cultured with induction media. Lastly, metabolic profiles of each system show considerable overlap between both differentiation methods but subtle differences which potentially give rise to their resultant phenotype can be ascertained. The study highlights how material and chemical alterations in the cellular microenvironment have wide ranging effects on resultant tissue type.

Original languageEnglish
Pages (from-to)6895
JournalScientific Reports
Issue number1
Early online date31 Jul 2017
Publication statusE-pub ahead of print - 31 Jul 2017


  • Journal Article


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