Improving TCSPC data acquisition from CMOS SPAD arrays

Nikola Krstajic; S. Poland; David Tyndall; Richard Walker; Simao Coelho; David Day-Uei Li; Justin Richardson; Simon Ameer-Beg; Robert Henderson

doi:10.1117/12.2032807

Improving TCSPC data acquisition from CMOS SPAD arrays

Nikola Krstajic, S. Poland, David Tyndall, Richard Walker, Simao Coelho, David Day-Uei Li, Justin Richardson, Simon Ameer-Beg, Robert Henderson

School of Engineering

Research output: Contribution to conference › Paper › peer-review

Abstract / Description of output

We present a digital architecture for fast acquisition of time correlated single photon counting (TCSPC) timestamps from
32×32 CMOS SPAD array. Custom firmware was written to select 64 pixels out of 1024 available for fast transfer of
TCSPC timestamps. Our 64 channel TCSPC is capable of acquiring up to 10 million TCSPC timestamps per second over
a USB2 link. We describe the TCSPC camera (Megaframe), camera interface to the PC and the microscope setup. We
characterize the Megaframe camera for fluorescence lifetime imaging (FLIM) including instrument response function,
time resolution and variability of both across the array. We show a fluorescence lifetime image of a plant specimen
(Convallaria majalis) from a custom-built multifocal multiphoton microscope. The image was acquired in 20 seconds
(with average timestamp acquisition rate of 4.7 million counts per second).

Original language	English
DOIs	https://doi.org/10.1117/12.2032807
Publication status	Published - 17 Jun 2013
Event	SPIE European Conference on Biomedical Optics - Munich, Germany Duration: 17 Jun 2013 → 21 Feb 2014

Conference

Conference	SPIE European Conference on Biomedical Optics
Country/Territory	Germany
City	Munich
Period	17/06/13 → 21/02/14

Access to Document

10.1117/12.2032807

http://proceedings.spiedigitallibrary.org/proceeding.aspx?articleid=1698558

MM-FLIM: Multiplexed multiphoton fluorescence lifetime microscopy
Henderson, R. & Ameer-Beg, S.
BBSRC
1/10/11 → 30/09/14
Project: Research

Cite this

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title = "Improving TCSPC data acquisition from CMOS SPAD arrays",

abstract = "We present a digital architecture for fast acquisition of time correlated single photon counting (TCSPC) timestamps from32×32 CMOS SPAD array. Custom firmware was written to select 64 pixels out of 1024 available for fast transfer ofTCSPC timestamps. Our 64 channel TCSPC is capable of acquiring up to 10 million TCSPC timestamps per second overa USB2 link. We describe the TCSPC camera (Megaframe), camera interface to the PC and the microscope setup. Wecharacterize the Megaframe camera for fluorescence lifetime imaging (FLIM) including instrument response function,time resolution and variability of both across the array. We show a fluorescence lifetime image of a plant specimen(Convallaria majalis) from a custom-built multifocal multiphoton microscope. The image was acquired in 20 seconds(with average timestamp acquisition rate of 4.7 million counts per second).",

author = "Nikola Krstajic and S. Poland and David Tyndall and Richard Walker and Simao Coelho and Li, {David Day-Uei} and Justin Richardson and Simon Ameer-Beg and Robert Henderson",

year = "2013",

month = jun,

day = "17",

doi = "10.1117/12.2032807",

language = "English",

note = "SPIE European Conference on Biomedical Optics ; Conference date: 17-06-2013 Through 21-02-2014",

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TY - CONF

T1 - Improving TCSPC data acquisition from CMOS SPAD arrays

AU - Krstajic, Nikola

AU - Poland, S.

AU - Tyndall, David

AU - Walker, Richard

AU - Coelho, Simao

AU - Li, David Day-Uei

AU - Richardson, Justin

AU - Ameer-Beg, Simon

AU - Henderson, Robert

PY - 2013/6/17

Y1 - 2013/6/17

N2 - We present a digital architecture for fast acquisition of time correlated single photon counting (TCSPC) timestamps from32×32 CMOS SPAD array. Custom firmware was written to select 64 pixels out of 1024 available for fast transfer ofTCSPC timestamps. Our 64 channel TCSPC is capable of acquiring up to 10 million TCSPC timestamps per second overa USB2 link. We describe the TCSPC camera (Megaframe), camera interface to the PC and the microscope setup. Wecharacterize the Megaframe camera for fluorescence lifetime imaging (FLIM) including instrument response function,time resolution and variability of both across the array. We show a fluorescence lifetime image of a plant specimen(Convallaria majalis) from a custom-built multifocal multiphoton microscope. The image was acquired in 20 seconds(with average timestamp acquisition rate of 4.7 million counts per second).

AB - We present a digital architecture for fast acquisition of time correlated single photon counting (TCSPC) timestamps from32×32 CMOS SPAD array. Custom firmware was written to select 64 pixels out of 1024 available for fast transfer ofTCSPC timestamps. Our 64 channel TCSPC is capable of acquiring up to 10 million TCSPC timestamps per second overa USB2 link. We describe the TCSPC camera (Megaframe), camera interface to the PC and the microscope setup. Wecharacterize the Megaframe camera for fluorescence lifetime imaging (FLIM) including instrument response function,time resolution and variability of both across the array. We show a fluorescence lifetime image of a plant specimen(Convallaria majalis) from a custom-built multifocal multiphoton microscope. The image was acquired in 20 seconds(with average timestamp acquisition rate of 4.7 million counts per second).

U2 - 10.1117/12.2032807

DO - 10.1117/12.2032807

M3 - Paper

T2 - SPIE European Conference on Biomedical Optics

Y2 - 17 June 2013 through 21 February 2014

ER -

Improving TCSPC data acquisition from CMOS SPAD arrays

Abstract / Description of output

Conference

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Projects

MM-FLIM: Multiplexed multiphoton fluorescence lifetime microscopy

Cite this