In silico and in vitro analysis of promoter regions of two exopolygalacturonase coding genes of Fusarium oxysporum f.sp radicis lycopersici and regulation in Saccharomyces cerevisiae

A. de las Heras, M. T. Gonzalez Jaen, B. Patino, F. J. Ruiz-Duenas, A. Villalvilla, C. Vazquez*

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Fusarium oxysporum f.sp. radicis lycopersici (FORL) causes an important disease in tomato characterized by cell wall degradation. The characterization of cell wall-degrading polygalacturonase enzymes and their regulation is crucial to gain knowledge on the role of these enzymes in pathogenesis. The objectives of this work were to perform an in silico and in vitro analyses of the upstream regions of two exopolygalacturonase (EXOPG) coding genes of FORL and to develop an easy and reliable system to perform the functional analysis of those promoters. The analysis of the upstream regions of pgx1 and pgx2 genes revealed differences in the distribution of regulatory motifs. These upstream regions were fused with the beta-galactosidase reporter gene in a Saccharomyces cerevisiae vector. The induction of the reporter gene was analyzed in response to different carbon sources and the results compared to the induction pattern of pgx1 and pgx2 genes in in vitro cultures of FORL.

Original languageEnglish
Title of host publicationCURRENT RESEARCH TOPICS IN APPLIED MICROBIOLOGY AND MICROBIAL BIOTECHNOLOGY
EditorsA MendezVilas
Place of PublicationSINGAPORE
PublisherWORLD SCIENTIFIC PUBL CO PTE LTD
Pages589-593
Number of pages5
ISBN (Print)978-981-283-754-7
Publication statusPublished - 2009
Event2nd International Conference on Environmental, Industrial and Applied Microbiology - Seville, Spain
Duration: 28 Nov 20071 Dec 2007

Conference

Conference2nd International Conference on Environmental, Industrial and Applied Microbiology
Country/TerritorySpain
Period28/11/071/12/07

Keywords

  • exopolygalacturonases
  • Fusarium oxysporum f.sp radicis lycopersici
  • Saccharomyces cerevisiae
  • promoters
  • regulation
  • CARBON CATABOLITE REPRESSION
  • ASPERGILLUS-NIDULANS
  • BOTRYTIS-CINEREA
  • SCLEROTINIA-SCLEROTIORUM
  • TRANSCRIPTION FACTOR
  • GALACTURONIC ACID
  • ENDOPOLYGALACTURONASE
  • EXPRESSION
  • YEAST
  • VIRULENCE

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