In silico and in vitro analysis of promoter regions of two exopolygalacturonase coding genes of Fusarium oxysporum f.sp radicis lycopersici and regulation in Saccharomyces cerevisiae

A. de las Heras; M. T. Gonzalez Jaen; B. Patino; F. J. Ruiz-Duenas; A. Villalvilla; C. Vazquez

In silico and in vitro analysis of promoter regions of two exopolygalacturonase coding genes of Fusarium oxysporum f.sp radicis lycopersici and regulation in Saccharomyces cerevisiae

A. de las Heras
, M. T. Gonzalez Jaen
, B. Patino
, F. J. Ruiz-Duenas
, A. Villalvilla
, C. Vazquez^*

^*Corresponding author for this work

School of Engineering

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

Fusarium oxysporum f.sp. radicis lycopersici (FORL) causes an important disease in tomato characterized by cell wall degradation. The characterization of cell wall-degrading polygalacturonase enzymes and their regulation is crucial to gain knowledge on the role of these enzymes in pathogenesis. The objectives of this work were to perform an in silico and in vitro analyses of the upstream regions of two exopolygalacturonase (EXOPG) coding genes of FORL and to develop an easy and reliable system to perform the functional analysis of those promoters. The analysis of the upstream regions of pgx1 and pgx2 genes revealed differences in the distribution of regulatory motifs. These upstream regions were fused with the beta-galactosidase reporter gene in a Saccharomyces cerevisiae vector. The induction of the reporter gene was analyzed in response to different carbon sources and the results compared to the induction pattern of pgx1 and pgx2 genes in in vitro cultures of FORL.

Original language	English
Title of host publication	CURRENT RESEARCH TOPICS IN APPLIED MICROBIOLOGY AND MICROBIAL BIOTECHNOLOGY
Editors	A MendezVilas
Place of Publication	SINGAPORE
Publisher	World Scientific
Pages	589-593
Number of pages	5
ISBN (Print)	978-981-283-754-7
Publication status	Published - 2009
Event	2nd International Conference on Environmental, Industrial and Applied Microbiology - Seville, Spain Duration: 28 Nov 2007 → 1 Dec 2007

Conference

Conference	2nd International Conference on Environmental, Industrial and Applied Microbiology
Country/Territory	Spain
Period	28/11/07 → 1/12/07

Keywords / Materials (for Non-textual outputs)

exopolygalacturonases
Fusarium oxysporum f.sp radicis lycopersici
Saccharomyces cerevisiae
promoters
regulation
CARBON CATABOLITE REPRESSION
ASPERGILLUS-NIDULANS
BOTRYTIS-CINEREA
SCLEROTINIA-SCLEROTIORUM
TRANSCRIPTION FACTOR
GALACTURONIC ACID
ENDOPOLYGALACTURONASE
EXPRESSION
YEAST
VIRULENCE

Cite this

de las Heras, A., Gonzalez Jaen, M. T., Patino, B., Ruiz-Duenas, F. J., Villalvilla, A., & Vazquez, C. (2009). In silico and in vitro analysis of promoter regions of two exopolygalacturonase coding genes of Fusarium oxysporum f.sp radicis lycopersici and regulation in Saccharomyces cerevisiae. In A. MendezVilas (Ed.), CURRENT RESEARCH TOPICS IN APPLIED MICROBIOLOGY AND MICROBIAL BIOTECHNOLOGY (pp. 589-593). World Scientific.

de las Heras, A. ; Gonzalez Jaen, M. T. ; Patino, B. et al. / In silico and in vitro analysis of promoter regions of two exopolygalacturonase coding genes of Fusarium oxysporum f.sp radicis lycopersici and regulation in Saccharomyces cerevisiae. CURRENT RESEARCH TOPICS IN APPLIED MICROBIOLOGY AND MICROBIAL BIOTECHNOLOGY. editor / A MendezVilas. SINGAPORE : World Scientific, 2009. pp. 589-593

@inproceedings{4b3dd9e58d7847b2ae645ab247bcf929,

title = "In silico and in vitro analysis of promoter regions of two exopolygalacturonase coding genes of Fusarium oxysporum f.sp radicis lycopersici and regulation in Saccharomyces cerevisiae",

abstract = "Fusarium oxysporum f.sp. radicis lycopersici (FORL) causes an important disease in tomato characterized by cell wall degradation. The characterization of cell wall-degrading polygalacturonase enzymes and their regulation is crucial to gain knowledge on the role of these enzymes in pathogenesis. The objectives of this work were to perform an in silico and in vitro analyses of the upstream regions of two exopolygalacturonase (EXOPG) coding genes of FORL and to develop an easy and reliable system to perform the functional analysis of those promoters. The analysis of the upstream regions of pgx1 and pgx2 genes revealed differences in the distribution of regulatory motifs. These upstream regions were fused with the beta-galactosidase reporter gene in a Saccharomyces cerevisiae vector. The induction of the reporter gene was analyzed in response to different carbon sources and the results compared to the induction pattern of pgx1 and pgx2 genes in in vitro cultures of FORL.",

keywords = "exopolygalacturonases, Fusarium oxysporum f.sp radicis lycopersici, Saccharomyces cerevisiae, promoters, regulation, CARBON CATABOLITE REPRESSION, ASPERGILLUS-NIDULANS, BOTRYTIS-CINEREA, SCLEROTINIA-SCLEROTIORUM, TRANSCRIPTION FACTOR, GALACTURONIC ACID, ENDOPOLYGALACTURONASE, EXPRESSION, YEAST, VIRULENCE",

author = "\{de las Heras\}, A. and \{Gonzalez Jaen\}, \{M. T.\} and B. Patino and Ruiz-Duenas, \{F. J.\} and A. Villalvilla and C. Vazquez",

year = "2009",

language = "English",

isbn = "978-981-283-754-7",

pages = "589--593",

editor = "A MendezVilas",

booktitle = "CURRENT RESEARCH TOPICS IN APPLIED MICROBIOLOGY AND MICROBIAL BIOTECHNOLOGY",

publisher = "World Scientific",

address = "Singapore",

note = "2nd International Conference on Environmental, Industrial and Applied Microbiology ; Conference date: 28-11-2007 Through 01-12-2007",

}

de las Heras, A, Gonzalez Jaen, MT, Patino, B, Ruiz-Duenas, FJ, Villalvilla, A & Vazquez, C 2009, In silico and in vitro analysis of promoter regions of two exopolygalacturonase coding genes of Fusarium oxysporum f.sp radicis lycopersici and regulation in Saccharomyces cerevisiae. in A MendezVilas (ed.), CURRENT RESEARCH TOPICS IN APPLIED MICROBIOLOGY AND MICROBIAL BIOTECHNOLOGY. World Scientific, SINGAPORE, pp. 589-593, 2nd International Conference on Environmental, Industrial and Applied Microbiology, Spain, 28/11/07.

In silico and in vitro analysis of promoter regions of two exopolygalacturonase coding genes of Fusarium oxysporum f.sp radicis lycopersici and regulation in Saccharomyces cerevisiae. / de las Heras, A.; Gonzalez Jaen, M. T.; Patino, B. et al.
CURRENT RESEARCH TOPICS IN APPLIED MICROBIOLOGY AND MICROBIAL BIOTECHNOLOGY. ed. / A MendezVilas. SINGAPORE: World Scientific, 2009. p. 589-593.

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

TY - GEN

T1 - In silico and in vitro analysis of promoter regions of two exopolygalacturonase coding genes of Fusarium oxysporum f.sp radicis lycopersici and regulation in Saccharomyces cerevisiae

AU - de las Heras, A.

AU - Gonzalez Jaen, M. T.

AU - Patino, B.

AU - Ruiz-Duenas, F. J.

AU - Villalvilla, A.

AU - Vazquez, C.

PY - 2009

Y1 - 2009

N2 - Fusarium oxysporum f.sp. radicis lycopersici (FORL) causes an important disease in tomato characterized by cell wall degradation. The characterization of cell wall-degrading polygalacturonase enzymes and their regulation is crucial to gain knowledge on the role of these enzymes in pathogenesis. The objectives of this work were to perform an in silico and in vitro analyses of the upstream regions of two exopolygalacturonase (EXOPG) coding genes of FORL and to develop an easy and reliable system to perform the functional analysis of those promoters. The analysis of the upstream regions of pgx1 and pgx2 genes revealed differences in the distribution of regulatory motifs. These upstream regions were fused with the beta-galactosidase reporter gene in a Saccharomyces cerevisiae vector. The induction of the reporter gene was analyzed in response to different carbon sources and the results compared to the induction pattern of pgx1 and pgx2 genes in in vitro cultures of FORL.

AB - Fusarium oxysporum f.sp. radicis lycopersici (FORL) causes an important disease in tomato characterized by cell wall degradation. The characterization of cell wall-degrading polygalacturonase enzymes and their regulation is crucial to gain knowledge on the role of these enzymes in pathogenesis. The objectives of this work were to perform an in silico and in vitro analyses of the upstream regions of two exopolygalacturonase (EXOPG) coding genes of FORL and to develop an easy and reliable system to perform the functional analysis of those promoters. The analysis of the upstream regions of pgx1 and pgx2 genes revealed differences in the distribution of regulatory motifs. These upstream regions were fused with the beta-galactosidase reporter gene in a Saccharomyces cerevisiae vector. The induction of the reporter gene was analyzed in response to different carbon sources and the results compared to the induction pattern of pgx1 and pgx2 genes in in vitro cultures of FORL.

KW - exopolygalacturonases

KW - Fusarium oxysporum f.sp radicis lycopersici

KW - Saccharomyces cerevisiae

KW - promoters

KW - regulation

KW - CARBON CATABOLITE REPRESSION

KW - ASPERGILLUS-NIDULANS

KW - BOTRYTIS-CINEREA

KW - SCLEROTINIA-SCLEROTIORUM

KW - TRANSCRIPTION FACTOR

KW - GALACTURONIC ACID

KW - ENDOPOLYGALACTURONASE

KW - EXPRESSION

KW - YEAST

KW - VIRULENCE

M3 - Conference contribution

SN - 978-981-283-754-7

SP - 589

EP - 593

BT - CURRENT RESEARCH TOPICS IN APPLIED MICROBIOLOGY AND MICROBIAL BIOTECHNOLOGY

A2 - MendezVilas, A

PB - World Scientific

CY - SINGAPORE

T2 - 2nd International Conference on Environmental, Industrial and Applied Microbiology

Y2 - 28 November 2007 through 1 December 2007

ER -

de las Heras A, Gonzalez Jaen MT, Patino B, Ruiz-Duenas FJ, Villalvilla A, Vazquez C. In silico and in vitro analysis of promoter regions of two exopolygalacturonase coding genes of Fusarium oxysporum f.sp radicis lycopersici and regulation in Saccharomyces cerevisiae. In MendezVilas A, editor, CURRENT RESEARCH TOPICS IN APPLIED MICROBIOLOGY AND MICROBIAL BIOTECHNOLOGY. SINGAPORE: World Scientific. 2009. p. 589-593

In silico and in vitro analysis of promoter regions of two exopolygalacturonase coding genes of Fusarium oxysporum f.sp radicis lycopersici and regulation in Saccharomyces cerevisiae

Abstract

Conference

Keywords / Materials (for Non-textual outputs)

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Cite this