In situ characterization of leucocyte subpopulations after infection with Eimeria tenella in chickens

L Vervelde, AN Vermeulen, SHM Jeurissen

Research output: Contribution to journalArticlepeer-review

Abstract

We characterized the leucocyte subpopulations after infection with Eimeria tenella in both naive and immune chickens. Immunocytichemical staining was used to characterize the cells in situ, so that the interaction between host and parasite could be studied. More leucocytes were detected in the lamina propria of immune chickens, and leucocytes infiltrated the ceca more rapidly than in naive chickens, but the infiltration was less pronounced than in naive chickens. In naive chickens, most infiltrated leucocytes were macrophages and T cells. Two days after inoculation the number of CD4(+) cells had increased greatly. In immune chickens, mainly T cells (CD4(+) and CD8(+)) infiltrated the lamina propria, and in contrast to naive chickens, the number of CD8(+) cells exceeded the number of CD4(+) cells. Furthermore, we characterized which cells contained a parasite and which cells were detected next to the parasites, because these cells are probably involved in the arrested development of the parasites. In naive chickens, sporozoites were significantly more often located within or next to macrophages than in immune chickens. In immune chickens, sporozoites were significantly more often located, within or next to CD3(+), CD8(+), and TCR2(+) cells. In conclusion, the marked increase of CD4(+) cells after primary infection suggests that these cells are involved in the induction of the immune response, whereas the increase of CD8(+) cells after challenge infection suggests that these cells act as effector cells.

Original languageEnglish
Pages (from-to)247-256
Number of pages10
JournalParasite Immunology
Volume18
Issue number5
DOIs
Publication statusPublished - May 1996

Keywords

  • coccidiosis
  • IMMUNITY
  • LEUKOCYTES
  • gamma delta cells
  • COCCIDIOSIS
  • POSTNATAL-DEVELOPMENT
  • VERMIFORMIS
  • chicken
  • Eimeria
  • T-cytotoxic cells
  • CYTOKINES
  • LYMPHOID-TISSUES
  • T-helper cells
  • RESISTANCE
  • T-CELL RECEPTOR
  • MONOCLONAL-ANTIBODY

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